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化学学报
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化学学报 ›› 1988, Vol. 46 ›› Issue (2): 204-205. 上一篇    

研究论文

应用^3^1P NMR研究RNase A水解核糖核酸的机制

汪猷;徐耀忠;张伟君   

  1. 华东师范大学分析测试中心
  • 发布日期:1988-02-15

Study on mechanism of tribonucleic acid hydrolysis by ribonuclease A using ^3^1P NMR method

WANG YOU;XU YAOZHONG;ZHANG WEIJUN   

  • Published:1988-02-15

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474

关键词: 核糖核酸, 反应机理, 水解, 核磁共振, 核糖核酸酶

31P NMR spectroscopy was applied to study the reaction course of the hydrolysis of UpU by RNase A. For the 1st time, the individual changes in UpU, U>p (uridine 2',3'-cyclophosphate), and Up (uridine 3'-phosphate) were continuously monitored and quant. determine The 2-step mechanism is thus incontestably proved. The rate of transphosphorylation (k1) is calculated to be ~40.9 s-1, and the rate for the hydrolysis of U>p (k2) is 9.4 s-1.

Key words: RIBONUCLEIC ACID, REACTION MECHANISM, HYDROLYSIS, NUCLEAR MAGNETIC RESONANCE, RIBONUCLEASE

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