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化学学报
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化学学报 ›› 1989, Vol. 47 ›› Issue (3): 266-269. 上一篇    下一篇

研究论文

酶促合成肽的研究 II: 含羧基侧链氨基酸的肽的酶促合成

汪猷;冯冬梅;徐杰诚;陈玲玲   

  1. 中国科学院上海有机化学研究所
  • 发布日期:1989-03-15

Studies on enzymic synthesis of oligopeptides II: Thermolysin-catalyzed synthesis of peptides containing a side chain free carboxyl group

WANG YOU;FENG DONGMEI;XU JIECHENG;CHEN LINGLING   

  • Published:1989-03-15

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435

用嗜热菌酶酶促合成了一系列含门冬氨酸、谷氨酸的肽. 实验证明, 缩合反应中羧基组份中的门冬氨酸β-羧基或谷氨酸γ-羧基毋需保护. 同时也研究了邻近氨基酸残基对缩合反应的影响.

关键词: 氨基酸, 缩合反应, 谷氨酸, 天冬氨酸, 肽, 羧基, 酶促, 嗜热细菌

A no. of peptides were synthesized from the carboxyl component containing a free side chain carboxyl group of aspartyl or glutamyl residue through thermolysin catalysis. The effects of neighboring residues situated closely to a C-terminal glutamic or aspartic acid in the carboxyl component on the formation of peptide bond were studied. The efficiency of coupling by thermolysin varied with the nature of the amino component and the carboxyl component. Strict stereospecificity was observed

Key words: AMINO ACID, CONDENSATION REACTION, GLUTAMIC ACID, ASPARTIC ACID, PEPTIDE, CARBOXY GROUP, ENZYMATIC, THERMOPHILIC BACTERIA

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