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化学学报
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化学学报 ›› 1994, Vol. 52 ›› Issue (1): 83-88. 上一篇    下一篇

研究论文

流动注射化学发光免疫分析研究I. 血清中乙型肝炎表面抗原的测定

张新荣;封满良;吕九如;章竹君   

  1. 陕西师范大学化学系
  • 发布日期:1994-01-15

Studies on flow injection chemiluminescence immunoassay I. determination of HBsAg in serum

ZHANG XINRONG;FENG MANLIANG;LU JIURU;ZHANG ZHUJUN   

  • Published:1994-01-15

PDF

457

被引次数

8

我们首次以键合有抗体的多孔玻璃作为固相免疫分析的免疫反应器, 以化学发光作为最终检测手段, 建立了一种新的、高效率的免疫分析技术-流动注射化学发光免疫分析技术。实验表明: 采用该技术可使单次测定时间从ELISA(Enzyme-linked Immunosorbent Assay)法的二十多小时降至二十分钟, 且所有操作均可在微机控制下自动完成。用该方法对人血清中乙型肝炎表面抗原的测定结果与ELISA法所得结果一致, 对同一样品连续九次测定的相对标准偏差为7.2%。因此, 该方法具有自动化程度高、分析速度快、稳定性好的优点。

关键词: 肝炎, 血清, 流动注射分析, 化学发光, 表面抗原, 免疫分析

In this paper, a new immunoassay technique, flow Chemiluminescence injection immunoassay, in which controlleld pore glass immobilized with antibody was used as immunoreactor and chemiluminescence for end-point monitoring, has been developed. Researches show that the time required per assay only 20 min with this method while 20h with conventional ELISA. Moreover, the whole assay can been taken automatically under the control of a microprocessor. This technique has been used for the determination of HBsAg in serum, and the results are comparable with that of ELISA. In addition, it has a relative standard deviation of 7.2%(9 assays). So this technique offers advantages of automatically rapidly and stablity.

Key words: HEPATITIS, SERUM, FLOW INJECTION ANALYSIS, CHEMILUMINESSENCE, SURFACE ANTIGENS

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