关键词: 高速液体色谱, 络合作用, 蛋白质, 色谱, 热力学分析, 疏水作用, 保留模型

In this paper, on the basis of the principles of the preferential hyddration interaction, proceeded from thermodynamics, the retention model of protein on the high performance hydrophobic interaction chromatography (HPHIC or HIC) has been deduced. The relation among the model parameters established has caused the experimental laws observed in the past to have a theoretical bases to follow, and in the light of the above-mentioned models, some important chromatographic characteristics of some proteins on the hydrophobic interaction chromatography have been expounded.

Key words: HIGH SPEED LIQUID CHROMATOGRAPHY, COMPLEXATION, PROTEIN, CHROMATOGRAPHY, THERMODYNAMIC ANALYSIS