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化学学报
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化学学报 ›› 1997, Vol. 55 ›› Issue (2): 172-177. 上一篇    下一篇

研究论文

全反式球形烯的光敏异构化及异构体的光谱

姜月顺;小山泰   

  1. 吉林大学化学系;关西学院大学理学部
  • 发布日期:1997-02-15

Photosensitized isomerization of all-trans spheroidene and electronic absorption spectra of the isomers

JIANG YUESHUN;XIAO SHANTAI   

  • Published:1997-02-15

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被引次数

1

从Rhodobacter sphaeroides 2.4.1提取了全反式球形烯, 用高压液相色谱(HPLC)分离了它的I2增感异构化产物: 9,13'-cis(峰A), 5,13-cis+13,9'-cis(峰B), 13-cis(峰C), 5,13'-cis(峰E1), 9-cis(峰E2), 13'-cis(峰F),5,9'-cis(峰G), 9'-cis(峰H), all-trans(峰I), 研究了异构体的结构对其电子吸收光谱及HPLC保留时间的影响, 从而确认峰E1为5,13'-cis。对全反式球形烯的光敏异构化机理也作了初步探讨。

关键词: 光敏化, 反应机理, 高速液体色谱, 异构化, 球形烯

All-trans-spheroidene was extracted from the cells of Rhodobacter sphaeroides 2.4.1 and a set of cis-trans isomers [9,13'-cis (peak A), 5,13-cis+13,9'-cis (peak B), 13-cis (peak C), 5,13'-cis (peak E1), 9-cis (peak E2), 13'-cis (peak F), 5,9'-cis (peak G), 9'-cis (peak H), all-trans (peak I)] were separated from an isomeric mixture obtained by iodine-sensitized photo-isomerization of the all-trans isomer by high-pressure liquid chromatography (HPLC) using a calcium hydroxide column. The effect of isomeric structures on electronic absorption spectra and retention of HPLC was studied and peak E1 was identified as 5,13'-cis. The mechanism of photosensitized isomerization of all-trans spheroidene was also discussed.

Key words: PHOTOSENSITIZATION, REACTION MECHANISM, HIGH SPEED LIQUID CHROMATOGRAPHY, ISOMERIZATION

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