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化学学报
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化学学报 ›› 1998, Vol. 56 ›› Issue (1): 47-51. 上一篇    下一篇

研究论文

稀土元素对3'-腺嘌呤及3'-鸟嘌呤核苷酸的水解断裂作用

朱兵;李新民;吴亦洁;赵大庆;倪嘉缵   

  1. 中国科学院长春应用化学研究所;中国科学院稀土化学与物理开放实验室
  • 发布日期:1998-01-15

Hydrolytic cleavage of adenosine-3'-monophosphate and Guanosine-3'-monophosphate by Lanthanides

ZHU BING;LI XINMIN;WU YIJIE;ZHAO DAQING;NI JIAZUAN   

  • Published:1998-01-15

PDF

528

被引次数

9

用核磁共振(NMR)波谱、HPLC和化学定磷法研究了稀土元素对3'-腺嘌呤核苷酸(3'-AMP)3及3'-鸟嘌呤核苷酸(3'-GMP)的水解断裂作用, 结果表明: 不同稀土元素对3'位核苷酸断裂有较强的特异性, 在37℃, pH9的条件下, 使3'-AMP断裂为腺嘌呤核苷(A)及无机磷, 使3'-GMP断裂为鸟嘌呤核苷(G)及无机磷, 其断裂作用远大于5'位核苷酸, 并对其水解断裂进行了研究。

关键词: 水解, 核磁共振谱法, 鸟嘌呤, 腺嘌呤, 核苷酸

The hydrolytic cleavage of adenosine-3'-monophosphate (3'-AMP) and guanosine-3'-monophosphate (3-GMP) by lanthanides was investigated by NMR, HPLC and the method of measuring the liberated inorganic phosphate. The results show that lanthanides have specificity for hydrolyzing 3'-mononucleotides. 3'-AMP and 3'-GMP were converted to Adenosine(A), phosphate and Guanosine(G), phosphate respectively at pH 9.37℃. The efficiency of cleavage was greater than that of 5'-mononucleotides. The mechanism of hydrolytic of cleavage was discussed in detail.

Key words: HYDROLYSIS, NMR SPECTROMETRY, GUANINE, ADENINE, NUCLEOTIDES

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