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化学学报
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化学学报 ›› 1999, Vol. 57 ›› Issue (8): 914-921. 上一篇    下一篇

研究论文

OAP-H~2O~2-HRP伏安酶联免疫分析新体系测定人血清铁蛋白

张书圣;焦奎;陈洪渊   

  1. 南京大学化学系.南京(210008);青岛化工学院应用化学系.青岛(266042)
  • 发布日期:1999-08-15

OAH-H~2O~2-HRP voltammetric enzyme-linked immunoassay for trace ferritin in human serum

Zhang Shusheng;Jiao Kui;Chen Hongyuan   

  1. Nanjing Univ, Dept Chem.Nanjing(210008);Qindao Inst Chem Technol, Dept Appl Chem.Qingdao(266042)
  • Published:1999-08-15

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首次提出邻氨基酚(OAP)-H~2O~2-辣根过氧化物酶(HRP)伏安酶联免疫分析新体系并用于人血清中铁蛋白的测定.本方法以线性扫描二阶导数伏安法栓测HRP催化H~2O~2氧化OAP的产物,用于游离HRP和各种HRP标记物的测定,灵敏度均高于经典的ELISA显色光度法.测定游HPR的线性范围为1.0x10^-^1^2-4.0x10^-^9g/mL,检测限达6.0x10^-^1^3g/mL.本法对铁蛋白测定的线性范围为0.2-320ng/mL,用所建立的方法对人血清样品进行了测定,并与现行的ELISA显色光度法进行对照,二者相关性很好.对此伏安酶联免疫分析新体系的电极还原过程也进行了详细的研究.

关键词: 免疫测定, 铁蛋白, 氨基酚, 辣根过氧化物酶, 过氧化氢, 伏安法

A voltammetic enzyme-linked immunoassay based on new system of o- aminophenol (OAP)-H~2O~2-horseradish peroxidase (HRP) has firstly been developed and used for the detection of HRP and ferritin.HRP or labelled HRP catalyzes the oxidation reaction of OAP with H~2O~2, producing a sensitive voltammetric peak at -0.43V(vs.SCE) in Britton- Robinson(B-R) buffer solution.By using this voltammetric peak,HRP can be measured with a detection limit of6.0-10^-^1^3g/mL and a linear range of 1.0*10^-^1^2-4.0*10^-^9g/mL. The detection limit for ferritin is 0.2ng/mL and the linear range 0.2-320ng/mL. The detection limit for ferritin with this method is lower than that with the enzyme-linked immunosorbent spectrophotometric assay(ELISA).

Key words: IMMUNOASSAY, FERRITIN, AMINOPHENOL, HYDROGEN PEROXIDE, VOLTAMMETRY

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