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化学学报
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化学学报 ›› 2000, Vol. 58 ›› Issue (12): 1677-1680. 上一篇    下一篇

研究论文

^1^4C-加速器质谱法研究NNK与血红蛋白的体内加 合作用

王艳;王海芳;孙红芳;何龙;刘元方;刘克新;鲁向阳;马宏骥   

  1. 北京大学技术物理学系.北京(100871);北京大学重离子研究所
  • 发布日期:2000-12-15

Study of hemoglobin adduction with NNK in vivo by ^1^4C-AMS

Wang Yan;Wang Haifang;Sun Hongfang;He Long;Liu Yuanfang;Liu Kexin;Lu Xiangyang;Ma Hongji   

  1. Beijing Univ., Dept. of Technical Physics.Beijing(100871)
  • Published:2000-12-15

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用^1^4C-加速器质谱法(AMS)研究了香烟中尼古丁的亚硝基衍生物4-(甲基亚硝胺基)-1-(3-吡啶基)-1-丁酮(NNK)与小鼠体内血红蛋白(Hb)的加合作用，给出了加合剂量响应曲线。NNK剂量在0.01～0.1μg/kgb.w.范围内，加合水平基本上是一样的；而在0.1～3.0μg/kgb.w.范围内，加合物数量与NNK剂量之间呈1g/1g表示的线性关系。同时研究发现一次性给药的NNK-Hb加合物在小鼠体内的衰减表现为快、慢两个阶段，前3天内衰减很快，3天后衰减不明显。

关键词: 碳十四加速器质谱法, 血红蛋白, 加合物, 尼古丁

Mouse blood hemoglobin (Hb) adduction with 4-(methylnitrosamino)-1- (3-pyridyl)-1-butanone (NNK0 was studied by ^1^4C-accelerator mass spectrometry (^1^4C-AMS). The dose range of NNK administered is 0.01 ～3.0μg/kg b.w.. In the exposure of mice to NNK ranging from 0.1 to 3. 0μg/kg b.w., the number of Hb adducts increases with increasing dose elvels with linear relationship in 1g/1g presentation. However, the number of Hb adducts almost keeps no change over a dose range of 0. 01～0.1μg/kg b.w.. As the time elapses, the number of Hb adducts reduces quickly in the first 3 days and no marked decay appears afterwards.

Key words: HEMOGLOBIN, ADDITION COMPOUNDS, NICOTINE

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