化学学报 ›› 2002, Vol. 60 ›› Issue (9): 1656-1661. 上一篇    下一篇

研究论文

考马斯亮蓝显色剂变色反应机理的研究

曹稳根;焦庆才;刘茜;陈雷   

  1. 南京大学生命科学学院,南京(210093)
  • 发布日期:2002-09-15

Study on the Mechanism of Color Changes of Commassie Brilliant Blue G-250

Cao Wengen;Jiao Qingcai;Liu Qian;Chen Lei   

  1. Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University,Nanjing(210093)
  • Published:2002-09-15

应用光谱法研究了考马斯亮蓝G-250(CBBG)显色剂变色反应的机理,考察了 不同实验条件对CBBG显色剂吸收光谱的影响。研究结果表明,CBBG显色剂在反应体 系中呈现出从蓝色→绿色→棕红色等多种颜色变化,主要是由CBBG分子间疏水相互 作用形成的聚集体聚集程度的不同所引起的。

关键词: 考马斯亮蓝, 变色作用, 疏水性, 反应机理, 聚集体

The mechanism of color changes of Commassie Brilliant Blue G-250 (CBBG) has been investigated by spectrometry. The influence of different experimental conditions on the absorption spectra of CBBG was inspected. It is found that CBBG forms aggregate by hydrophobic interaction in high phosphoric acid concentration. Due to the difference in the aggregative degree, CBBG shows color changes from blue to green to brown in the system.

Key words: Commassie Brilliant Blue G-250, METAGENESIS, HYDROPHOBILITY, REACTION MECHANISM, AGGREGALES

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