SIOC English
化学学报
高级检索

化学学报 ›› 2004, Vol. 62 ›› Issue (10): 992-997. 上一篇    下一篇

研究论文

天然生物材料壳聚糖支架上人胚肺成纤维细胞的生长

陈光1, 周平1, 潘銮凤2, 梅娜1, 吴春根2, 陈新1, 邵正中1   

  1. 1. 复旦大学高分子科学系, 教育部聚合物分子工程重点实验室, 上海, 200433;
    2. 复旦大学上海医学院, 分子生物学实验室, 上海, 200032
  • 投稿日期:2003-08-22 修回日期:2004-01-20 发布日期:2014-02-17
  • 通讯作者: 周平,E-mail:pingzhou@fudan.edu.cn E-mail:pingzhou@fudan.edu.cn
  • 基金资助:
    国家自然科学基金(Nos.20274009,29974004)、复旦大学Med-X基金资助项目.

Growth of Human Fetal Lung Fibroblasts on the Natural Biomaterial-Chitosan Scaffold

CHEN Guang1, ZHOU Ping1, PAN Luan-Feng2, MEI Na1, WU Chun-Gen2, CHEN-Xin1, SHAO Zheng-Zhong1   

  1. 1. Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Macromolecular Science Department, Fudan University, Shanghai 200433;
    2. Laboratory of Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032
  • Received:2003-08-22 Revised:2004-01-20 Published:2014-02-17

PDF

429

被引次数

49

采用不同粒度的硅胶粒子作为致孔剂,按硅胶和壳聚糖重量比9∶1,制备了三组不同孔径的壳聚糖多孔支架.以无孔壳聚糖支架为参照,对多孔支架的有效孔径、吸水性进行了比较.结果表明:孔径大小由硅胶尺寸控制,吸水性随孔径增大而增大.为研究支架孔径大小对其生物相容性的影响,在系列支架上进行了人胚肺成纤维细胞的培养.细胞种植1 d后,多孔支架上的细胞粘附较多,而无孔支架上的细胞伸展情况较好;细胞培养5 d后,所有支架上细胞伸展情况良好,孔径越大的支架上细胞增殖越多.该研究结果将为天然生物材料壳聚糖作为组织工程支架材料的应用提供有益的指导.

关键词: 壳聚糖, 人胚肺成纤维细胞, 多孔支架, 组织工程

A series of porous chitosan scaffolds were fabricated with various sizes of silica particles as porogen at chitosan/silica weight ratio of 9∶1. The effective pore sizes and bibulous ability of the porous scaffolds were compared with those of non-porous films. It shows that the pore size is determined by the size of silica particles while the bibulous ratio is enhanced with increasing pore sizes. To evaluate their biocompatibility, human fetal lung fibroblasts were cultured on these scaffolds. The results show that all the scaffolds availed to the cell growth. After one day of cell seeding, the porous scaffolds held more viable cells than the non-porous did, while cells spread better on the control film. Five days later, the cell proliferation occurred on all the scaffolds, whereas considerably more cells proliferated on the porous ones, and the bigger the pore size was, the faster cell proliferation was achieved. These results would contribute to the application of chitosan scaffolds in tissue engineering.

Key words: chitosan, human fetal lung fibroblast, porous scaffold, tissue engineering