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化学学报
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化学学报 ›› 2004, Vol. 62 ›› Issue (15): 1379-1384. 上一篇    下一篇

研究论文

聚丙烯片基不同气氛下等离子体改性及DNA原位合成研究

汤建新1,2, 周灵君3, 陈洪1, 何农跃1,2,4   

  1. 1. 株洲工学院包装新材料与技术重点实验室, 株洲, 412008;
    2. 东南大学教育部分子与生物分子电子学重点实验室, 南京, 210096;
    3. 南京中医药大学药学院, 南京, 210029;
    4. 南京医科大学第一附属医院, 南京, 210029
  • 投稿日期:2004-01-13 修回日期:2004-04-02 发布日期:2014-02-17
  • 通讯作者: 何农跃,E-mail:nyhe@seu.edu.cn;Fax:025-83619983. E-mail:nyhe@seu.edu.cn
  • 基金资助:
    国家863计划(Nos.102-01-01,2003AA302270),国家自然科学基金(No.60071001),教育部跨世纪优秀人才培养计划基金(教财司函[2003]36号)和中国博士后科学基金(2002)资助项目.

A Comparative Study on in situ Synthesis of Oligonucleotide on Different Plasma Modified Polypropylene Surfaces

TANG Jian-Xin1,2, ZHOU Ling-Jun3, CHEN Hong1, HE Nong-Yue1,2,4   

  1. 1. Key Laboratory for Packing Materials and New Technology of Zhuzhou Institute of Technology, Zhuzhou 412008;
    2. Key Laboratory for Molecular and Biomolecular Electronics of Ministry of Education, Southeast University, Nanjing 210096;
    3. College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210029;
    4. First Affiliated Hospital, Nanjing Medical University, Nanjing 210029
  • Received:2004-01-13 Revised:2004-04-02 Published:2014-02-17

PDF

399

被引次数

5

分别采用氮气/氢气、氨气和氧气三种不同气氛的等离子体处理了聚丙烯片基,先使其表面接枝功能性基团,然后分别进行寡核苷酸原位合成.光电子能谱(XPS)证实了在其表面分别接枝了大量氨基和其它含氮基团.荧光扫描分析并比较了在三种方法处理的聚丙烯片基上合成的寡核苷酸与靶序列杂交后的荧光强度.结果表明:三种方法处理的聚丙烯片基都可用于DNA原位合成,但从处理工艺和荧光分析结果来看,以氮气/氢气等离子体处理的聚丙烯片基最佳.

关键词: 等离子体, 聚丙烯, 寡核苷酸探针阵列, 原位合成

This paper compared results of oligonucleotide probe array in-situ synthesized on the hydrogen and nitrogen plasma modified polypropylene (PP) surface with those on the amino plasma modified PP surface and also with those on oxygen plasma and silanizing modified PP surface.X-ray photoelectron spectroscopy (XPS) analysis confirmed the presence of polar amino groups on the oxygen plasma and silanizing treated PP surfaces.The specificity of the probe arrays was discriminated by hybridizing with fluorescent target sequences.The results indicated that the three different plasma-functionalized PP surfaces all could serve as good substrates for high-density oligonucleotide array synthesis and DNA chip fabrication.However, the hydrogen and nitrogen plasma-functionalized PP surface was the best.

Key words: plasma, polypropylene, oligonucleotide probe array, in-situ synthesis