化学学报 ›› 2004, Vol. 62 ›› Issue (20): 2118-2122. 上一篇    下一篇

研究简报

巯基苯类为标记分子免疫检测的FT-SERS光谱研究

李淑瑾, 顾仁敖   

  1. 苏州大学化学系, 苏州, 215006
  • 投稿日期:2004-03-03 修回日期:2004-05-25 发布日期:2014-02-17
  • 通讯作者: 顾仁敖,E-mail:ragu@suda.edu.cn E-mail:ragu@suda.edu.cn
  • 基金资助:
    国家自然科学基金(Nos.20273045,20373046)和厦门大学固体表面物理化学国家重点实验室开放基金资助项目.

Study on Fourier Transform Surface-Enhanced Raman Scattering of Immunoassay Using Mercapto Molecules as Reporter-labels

LI Shu-Jin, GU Ren-Ao   

  1. Department of Chemistry, Suzhou University, Suzhou 215006
  • Received:2004-03-03 Revised:2004-05-25 Published:2014-02-17

以对巯基苯甲酸、苯硫酚为标记分子,与金纳米粒子生成具有SERS信号的标记金溶胶.在一定条件下,标记金溶胶与抗体羊抗小鼠IgG形成SERS标记免疫金溶胶.用组装金纳米粒子的玻璃表面吸附抗体,通过对相应抗原小鼠IgG的识别,形成固相抗体/抗原对,再将SERS标记免疫金溶胶组装到固相抗体/抗原对上,形成"固相抗体-待测抗原-标记抗体(即标记免疫金溶胶)"夹心复合物,进而通过拉曼标记分子的SERS信号进行免疫检测.

关键词: 对巯基苯甲酸, 苯硫酚, 表面增强拉曼散射, 金纳米粒子, 免疫检测

Gold colloids were labeled with Raman reporter mercapto molecules such as 4-mercaptobenzoic acid and thiophenol to form Fourier transform (FT) surface-enhanced Raman scattering (SERS) reporter-labeled gold colloids. Firstly, antibody goat anti-rat was adsorbed onto the reporter-labeled gold colloids to form reporter-labeled immunogold colloids in appropriate condition. Secondly, the glass slide which was assembled gold nanoparticles was coated with antibody and selectively adsorbed antigen rat IgG to form antibody-antigen couple, and the SERS reporter-labeled immunogold colloids were modified onto the couple in a glass slide with gold nanoparticles adsorbing "antibody/antigen/reporter-labeled immunogold colloids" to form sandwich-type complex through the specific adsorption between antibody and antigen. The selective interaction between antibody and antigen can be read out by the labeling molecules.

Key words: 4-mercaptobenzoic acid, thiophenol, surface enhanced Raman scattering, gold nanoparticle, immunoassay