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化学学报
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化学学报 ›› 2004, Vol. 62 ›› Issue (9): 923-928. 上一篇    

研究论文

微波辐射-酶耦合催化(MIECC)戊醇同分异构体与n-辛酸的酯化反应

蔡汉成1, 高惠2, 方银军2, 夏咏梅1, 蒋陈兰1, 方云1   

  1. 1. 江南大学化学与材料工程学院,无锡,214036;
    2. 浙江省轻工业研究所,杭州,310009
  • 投稿日期:2003-08-15 修回日期:2003-12-20 发布日期:2014-02-17
  • 通讯作者: 方云,E-mail:fangyunzhou@hotmail.com E-mail:fangyunzhou@hotmail.com
  • 基金资助:
    江苏省自然科学基金(No.BJ99036)及浙江省赞成科学研究基金(No.ZC200301)资助项目.

Esterification between Amyl Alcohol Isomers and n-Caprylic Acid under Microwave Irradiation-Enzyme Coupling Catalysis (MIECC)

CAI Han-Cheng1, GAO Hui2, FANG Ying-Jun2, XIA Yong-Mei1, JIANG Chen-Lan1, FANG Yun1   

  1. 1. School of Chemical and Material Engineering, Southern Yangtze University, Wuxi 214036;
    2. Zhejiang Institute of Light Industry, Hangzhou 310009
  • Received:2003-08-15 Revised:2003-12-20 Published:2014-02-17

PDF

654

被引次数

42

以戊醇同分异构体与n-辛酸为反应底物,比较在三种模式下进行的非水相酶催化酯化反应:(1)在线微波辐射(MI)模式;(2)常规加热(CH)模式;(3)离线微波预辐射酶+常规加热(pre-MI+CH)模式,以揭示微波辐射-酶耦合催化(MIECC)的耦合效应.实验结果表明,MIECC在提高酯化反应初速率方面表现出明显的耦合效应,可加快反应初速率2.5～4.5倍.醇结构对MIECC有影响,在反应初速率及平衡产率方面均遵循伯醇＞仲醇＞叔醇的规律.MI模式或pre-MI+CH模式对酶均有激活作用,经微波预辐射的酶产生某种记忆效应,酶能持久性地被改变催化活性.

关键词: 微波辐射, 酶催化, 酯化

The enzymatic esterification between amyl alcohol isomers and n-caprylic acid was investigated in the following three ways to reveal the potential coupling mechanism of microwave irradiation enzyme coupling catalysis (MIECC).That is, (1) MI manner: on line microwave irradiation with 2450 MHz emission frequency and constant output power, (2) CH manner: conventional isothermal heating and (3) pre-MI+CH manner: enzyme pre-treated by microwave irradiation followed by catalysis under conventional heating.The obvious MIECC effect on increasing initial reaction rate 2.5～4.5 times but less influence on shifting reaction equilibrium was observed.The structure of amyl alcohol affects both the initial reaction rate and the ultimate yield of amyl n-caprylate in the following sequence: primary alcoholsecondary alcoholtertiary alcohol.Increment of microwave power shows some effect on the initial reaction rate of esterification but the shot intermittence of MI/cooling seems more favorable to MIECC in reaction rate.The experimental results show that no matter how MI or pre-MI+CH manner can activate enzymes and especially the enzyme that undergoes pre-MI can keep the stimulated activity during following reaction due to some memory function of enzyme to pre-MI.

Key words: microwave irradiation, enzymatic catalysis, esterification