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化学学报
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化学学报 ›› 2005, Vol. 63 ›› Issue (22): 2093-2097. 上一篇    下一篇

研究论文

纳米金聚集复合物放大的计时电位法测定补体C3

周谷珍1,2，李继山1，蒋健晖1，沈国励*,1，俞汝勤1   

  1. (1湖南大学化学化工学院 化学生物传感与计量学国家重点实验室 长沙 410082)
    (2湖南文理学院化学化工系 常德 415000)
  • 投稿日期:2005-03-09 修回日期:2005-07-29 发布日期:2010-12-10
  • 通讯作者: 沈国励

Chronopotentiometry Based on Nano-Au Labeled Aggregate Enlargement Used for the Immunoassay of Complement C3

ZHOU Gu-Zhen1,2, LI Ji-Shan1, JIANG Jian-Hui1, SHEN Guo-Li*,1, YU Ru-Qin1   

  1. (1 State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,
    Hunan University, Changsha 410082)
    (2 Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000)
  • Received:2005-03-09 Revised:2005-07-29 Published:2010-12-10
  • Contact: SHEN Guo-Li

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报道了一种用金标复合物放大的计时电位法测定补体C3的电化学免疫检测方法. 对影响传感器响应的因素如蛋白A固定浓度、抗体包被过程的pH及浓度、金标物混合比等进行了考察. 在优化的实验条件下, 传感器的信号响应和补体C3的浓度在0.12～117.3 ng/mL范围内具有良好的线性关系, 检出限达0.02 ng/mL.

关键词: 计时电位法, 补体C3, 纳米金, 免疫分析

A novel electrochemical immunoassay method based on Au nanoparticle labeled immunocomplex enlargement used for detection of complement C3 was proposed in this paper. When the aggregates formed from nano-Au labeled goat-anti-human C3 and nano-Au labeled rabbit-anti-goat IgG were immobilized on the electrode surface by sandwich method (antibody/antigen/aggregate), the electrochemical signal of the electrode was enlarged greatly. Fabrication of the immunoelectrode and the effects of experimental conditions on the performances of immunoassay, such as concentration of protein A, pH of incubating solution and the ratio of nano-Au labeled goat-anti-human C3 to nano-Au labeled rabbit-anti-goat IgG, were discussed respectively. The formed immunosensor could quantitatively determine complement C3 in the range of 0.12～117.3 ng/mL, and the detection limit was 0.02 ng/mL.

Key words: chronopotentiometry, complement C3, nano-Au, immunoassay