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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (6): 537-542. 上一篇    下一篇

研究论文

一种基于电洗脱的核酸纯化回收芯片的设计及优化

冉瑞，李刚，赵辉，刘康栋，金庆辉，杨梦苏，赵建龙*   

  1. (1中国科学院上海微系统与信息技术研究所 上海 200050)
    (2中国科学院研究生院 北京100049)
    (3生物芯片上海国家工程研究中心 上海 201203)
  • 投稿日期:2005-07-12 修回日期:2005-11-30 发布日期:2006-03-28
  • 通讯作者: 赵建龙

Design and Optimization of a Chip Based on Electroelution for the Purification and Recovery of DNA Fragments

RAN Rui, LI Gang, ZHAO Hui, LIU Kang-Dong, JIN Qing-Hui, YANG Meng-Su, ZHAO Jian-Long*   

  1. (1 Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050)
    (2 Graduate School of Chinese Academy of Sciences, Beijing 100049)
    (3 National Engineering Center for Biochip at Shanghai, Shanghai 201203)
  • Received:2005-07-12 Revised:2005-11-30 Published:2006-03-28
  • Contact: ZHAO Jian-Long

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752

被引次数

1

提出了一种基于电洗脱原理的核酸纯化回收芯片, 通过对芯片上电极进行适当的切换操作, 可一次完成核酸样品分离和纯化回收. 同时采用数值模拟的方法对纯化回收芯片管道的几何形状及电场分布进行了优化设计, 并进行了实验验证. 实验结果与模拟分析非常吻合, 证明优化设计达到了预期的效果.

关键词: 核酸, 纯化, 回收, 数值模拟

A chip based on electroelution principle was presented for the recovery of DNA fragments, which can make the isolation and collection of target DNA fragments possible in a single process by switching electrodes. An optimization design was made for the channel structure and the distribution of the electric field in DNA recovery chip, and experiments were also performed to validate the simulation result. Both theoretical analysis and experimental results show that the optimization design in our existing device can achieve the desired results.

Key words: nucleic acid, purification, recovery, numerical simulation