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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (9): 917-922. 上一篇    下一篇

研究论文

[Cr(III)(SSA)(en)2]•2H2O配合物的合成、表征及性质研究

刘斌，李英奇，杨斌盛*   

  1. (山西大学分子科学研究所 化学生物学与分子工程教育部重点实验室 太原 030006)
  • 投稿日期:2005-08-05 修回日期:2006-01-12 发布日期:2006-05-15
  • 通讯作者: 杨斌盛

Synthesis, Characterization and Properties of Chromium(III) Complex [Cr(III)(SSA)(en)2]•2H2O

LIU Bin, LI Ying-Qi, YANG Bin-Sheng*   

  1. (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of China, Institute of Molecular Science, Shanxi University, Taiyuan 030006)
  • Received:2005-08-05 Revised:2006-01-12 Published:2006-05-15
  • Contact: YANG Bin-Sheng

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有机铬(III)配合物具有较高的生物利用率. 本文合成了一种新型磺基水杨酸铬(III)混配配合物[Cr(SSA)(en)2]•2H2O (SSA＝5-磺基水杨酸, en＝乙二胺), 通过红外、紫外、荧光光谱以及元素分析、电导率测定和X晶体衍射等方法对其结构进行了表征. 在pH 7.4, 0.05 mol•L－1 Tris-HCl缓冲液中, 利用荧光光谱研究了配合物与人血清白蛋白的结合. 结果表明配合物可与人血清白蛋白以较强的分子间作用力结合, 条件结合常数为(2.7±0.1)×104 mol•L－1, 结合位点数为3.87. 在pH 7.4, 0.05 mol•L－1 Tris-HCl缓冲液中, 观察了不同温度下EDTA和脱铁伴清蛋白为竞争剂的配体取代反应动力学行为, 其中37 ℃时反应速率常数分别为0.0142和0.0225 h－1.

关键词: 铬(III)配合物, 5-磺基水杨酸, 人血清白蛋白, 脱铁伴清蛋白, 荧光光谱

In this paper new chromium(III) complex [Cr(SSA)(en)2]•2H2O (en＝ethylenediamine, SSA＝5-sulfosalicylic acid) was synthesized and characterized by elemental analysis, IR, UV-Vis and X-ray crystallography. The interaction of human serum albumin with the complex was investigated by fluorescence spectra, the conditional binding constant K (2.7±0.1)×104 and the number of binding sites n (3.87) were obtained. The kinetics of release of Cr3＋from [Cr(SSA)(en) 2]•2H2O to EDTA and apoovotransferrin was investigated at different temperatures in 0.05 mol•L－1 Tris-HCl buffer at pH 7.4 by fluorescence spectrophotometer, and the respective rate constants at 37 ℃ were 0.0142 and 0.0225 h－1.

Key words: chromium(III) complex, 5-sulfosalicylic acid, human serum albumin, apoovotransferrin, fluorescence spectroscopy