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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (10): 1163-1167. 上一篇    下一篇

研究论文

Au改性TiO2纳米复合物对人结肠癌细胞的光催化杀伤作用

许娟1,2，陈智栋1，孙毅2，陈春妹3，江志裕*,2   

  1. (1江苏工业学院化学工程系 常州 213164)
    (2复旦大学化学系 分子催化与新材料重点实验室 上海 200433)
    (3复旦大学遗传学研究所 上海 200433)
  • 投稿日期:2007-10-17 修回日期:2007-12-01 发布日期:2008-05-28
  • 通讯作者: 江志裕

Photocatalytic Killing Effect of Gold-doped TiO2 Nanocomposites on Human Colon Carcinoma LoVo Cells

XU, Juan1,2, CHEN, Zhi-Dong1, SUN, Yi2, CHEN, Chun-Mei3, JIANG, Zhi-Yu*,2   

  1. (1 Department of Chemical Engineering, Jiangsu Polytechnic University, Changzhou 213164)
    (2 Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433)
    (3 Institute of Genetics, Fudan University, Shanghai 200433)
  • Received:2007-10-17 Revised:2007-12-01 Published:2008-05-28
  • Contact: JIANG, Zhi-Yu

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提出了通过TiO2表面修饰纳米Au的方法来提高纳米TiO2光催化杀伤癌细胞的效率. 采用化学还原法合成了Au改性的TiO2 (Au/TiO2)纳米复合物, 并研究了不同掺杂量(1 wt%, 2 wt%, 4 wt%)的Au/TiO2对人结肠癌LoVo细胞的光催化杀伤效应. 结果显示, Au的掺杂大大地提高了TiO2纳米粒子光催化杀伤结肠癌LoVo细胞的效率, 而且Au掺杂量的高低影响Au/TiO2光催化杀伤癌细胞的效率, 掺金量为2%的Au/TiO2对结肠癌LoVo细胞具有最高的光催化杀伤效率. 在光强为1.8 mW/cm2的紫外灯(λmax＝365 nm)下光照110 min, 50 μg/mL掺金量为2%的Au/TiO2能够杀死所有的癌细胞, 而同样浓度的TiO2只能杀死70%的癌细胞.

关键词: TiO2, Au/TiO2纳米复合物, 人结肠癌LoVo细胞, 光催化杀伤效率

Gold-doped TiO2 (Au/TiO2) nanocomposites were utilized to improve the photocatalytic inactivation effect on human colon carcinoma LoVo cells. The Au/TiO2 samples doped with different amounts of Au (1 wt%, 2 wt%, 4 wt%) were prepared by a chemical reduction method and used to photokill the LoVo cells. It was found that the photocatalytic inactivation effect of the TiO2 nanoparticles on LoVo cancer cells could be greatly improved by the surface modification of Au nanoparticles. Furthermore, the loading amount of Au on the surface of the TiO2 nanoparticles affected the photocatalytic inactivation efficiency strongly, and it was observed that the most efficient sample was 2 wt% Au/TiO2. Under the irradiation of UV light (λmax＝365 nm, intensity＝1.8 mW/cm2) for 110 min, all of the LoVo cancer cells were killed by 50 μg/mL 2 wt% Au/TiO2. But for 50 μg/mL TiO2 nanoparticles, only 70% cancer cells could be killed under the same condition.

Key words: TiO2, Au/TiO2 nanocomposite, human colon carcinoma LoVo cell, photocatalytic killing effect