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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (16): 1919-1923. 上一篇    下一篇

研究论文

纳米羟基磷灰石/丝素蛋白复合支架材料的降解特性及生物相容性研究

刘 琳a 孔祥东b 蔡玉荣a 姚菊明*,a

  

  1. (a浙江理工大学材料与纺织学院， 教育部先进纺织材料与制备技术重点实验室， 杭州 310018)
    (b浙江理工大学生命科学学院， 杭州 310018)
  • 投稿日期:2007-12-30 修回日期:2008-04-21 发布日期:2008-08-28
  • 通讯作者: 姚菊明

Degradation Behavior and Biocompatibility of Nano- hydroxyapatite/Silk Fibroin Composite Scaffolds

LIU, Lin a KONG, Xiang-Dong b CAI, Yu-Rong a YAO, Ju-Ming *,a   

  1. (a The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education,
    College of Materials and Textile, Zhejiang Sci-Tech University, Hangzhou 310018)
    (b College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018)
  • Received:2007-12-30 Revised:2008-04-21 Published:2008-08-28
  • Contact: YAO, Ju-Ming

PDF

1583

被引次数

51

应用共混法制备了纳米羟基磷灰石/丝素蛋白复合支架材料, 通过体外降解和细胞培养实验研究了复合支架材料的降解特性和生物相容性. 体外降解实验结果显示, 复合支架材料具有稳定的降解能力; 在降解过程中, 羟基磷灰石由于与降解液发生钙、磷等离子的交换, 使其结晶得到了进一步生长和完善. 利用细胞计数法、四甲基偶氮唑盐(MTT)比色法和碱性磷酸酶(ALP)活性测定等分析了复合支架材料的生物相容性, 结果表明, MG63细胞在复合支架材料上具有良好的粘附、增殖能力, 并可引起早期的骨分化. 因此, 纳米羟基磷灰石/丝素蛋白复合支架作为骨组织工程的支架材料具有良好的应用前景.

关键词: 羟基磷灰石, 丝素蛋白, 复合支架, 体外降解, 生物相容性

The nano-hydroxyapatite/silk fibroin (nHA/SF) composite scaffolds were prepared by the commixing process. The, and their in vitro degradation behavior and the biocompatibility was of nHA/SF composite scaffolds were investigated in this work. The in vitro degradation experiments showed that the composite scaffolds had a stable and appropriate degradation rate, accompanied by the HA crystal growth and perfection due to the ion exchanges of Ca2＋and between the HA crystals and the degradation buffer. The viable cell counting, methyl thiazolyl and tetrazolium (MTT) assay and alkaline phosphatase (ALP) test were used to evaluate the cell biocompatibility of the nHA/SF composite scaffolds. The data showed that the MG63 cells have had good adhesion and proliferation abilities on the nHA/SF composite scaffolds, which could induce the early osteogenic differentiation. ConclusionlyConclusively, the nHA/SF composite scaffolds may be a promising candidate for the bone tissue engineering.

Key words: hydroxyapatite (HA), silk fibroin, composite scaffold, in vitro degradation, biocompatibility