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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (4): 443-448. 上一篇    下一篇

研究论文

亚甲基蓝与鲱鱼精DNA相互作用的光谱法研究

费丹1, 王兴明*,1, 黎泓波1, 丁立生2, 胡亚敏1, 张欢1, 赵仕林3   

  1. (1西南科技大学材料科学与工程学院化学系 绵阳 621010)
    (2中国科学院成都生物研究所 成都 610041)
    (3四川师范大学化学学院 成都 610066)
  • 投稿日期:2007-01-29 修回日期:2007-07-25 发布日期:2008-02-28
  • 通讯作者: 王兴明

Spectroscopy Studies of Interaction between Methylene Blue and Herring Sperm DNA

FEI Dan1; WANG Xing-Ming*,1; LI Hong-Bo1; DING Li-Sheng2; HU Ya-Min1; ZHANG Huan1; ZHAO Shi-Lin3   

  1. (1 Department of Chemistry, School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010)
    (2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041)
    (3 College of Chemistry, Sichuan Normal University, Chengdu 610066)
  • Received:2007-01-29 Revised:2007-07-25 Published:2008-02-28
  • Contact: WANG Xing-Ming

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被引次数

73 | 9

以吖啶橙(AO)作为光谱探针, 采用UV和荧光光谱等方法研究了亚甲基蓝(MB)与鲱鱼精DNA的作用机制. 确定了在低浓度MB时, MB与DNA以嵌插方式作用; 而在高浓度MB时, MB与DNA之间为混合作用方式. 结合比n(MB)∶n(DNA)＝10∶1, 结合常数 ＝2.46×105 L•mol－1, MB-DNA复合物的表观摩尔吸光系数ε＝5.70×106 L•mol－1•cm－1. 同时研究了酸度和温度等对MB与DNA相互作用的影响, 热力学研究推导了MB结合DNA为焓驱动反应.

关键词: 亚甲基蓝, 吖啶橙, 鲱鱼精DNA, 作用方式

The UV-VIS spectroscopy and fluorescence spectroscopy were applied to investigate the mechanism of the interaction between methylene blue (MB) and herring sperm DNA by using acridine orange as a spectral probe in Tris-HCl buffer (pH 7.0). The chief action mode was comfirmed to be an intercalation binding at a lower MB concentration but the mixed action modes at a higher concentration. Combining ratio of the binary complex is n(MB)∶n(DNA)＝10∶1, the binding constant of MB with DNA is ＝2.46×105 L•mol－1 and the apparent molor absorptivity is ε＝5.70×106 L•mol－1•cm－1. Meanwhile, the interaction of MB with DNA was effected by the changes of pH and temperature. The results deduce that the interaction between MB and DNA is an enthalpy driven reaction.

Key words: methylene blue, acridine orange, herring sperm DNA, action mode