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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (16): 1923-1928. 上一篇    下一篇

研究论文

基于聚2,6-二氨基吡啶膜及纳米金修饰的癌胚抗原免疫传感器研究

管 姝 袁 若* 柴雅琴 唐点平 刘凯歌 王晋芬

  

  1. (西南大学化学化工学院发光与实时分析教育部重点实验室 重庆 400715)

  • 投稿日期:2008-09-27 修回日期:2009-01-16 发布日期:2009-08-28
  • 通讯作者: 袁若

Amperometric Immunosensor for Carcinoembryonic Antigen Based on Poly(2,6-pyridinediamine) Membrane and Gold Nanoparticles

Guan, Shu Yuan, Ruo* Chai, Yaqin Tang, Dianping Liu, Kaige Wang, Jinfen

  

  1. (Key Laboratory of Luminescence and Real-time Analysis of MOE, College of Chemical Engineering,
    Southwest China University, Chongqing 400715)
  • Received:2008-09-27 Revised:2009-01-16 Published:2009-08-28
  • Contact: Yuan Ruo

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1621

被引次数

32

在玻碳电极表面电聚合2,6-二氨基吡啶(pPA), 利用硫堇(Thi)、纳米金(nano-Au)固载癌胚抗体, 制得稳定性好、灵敏度较高、线性范围宽的电流型免疫传感器. 通过循环伏安法考察了该免疫传感器的电化学特性, 在优化的实验条件下, 该免疫传感器的峰电流随着检测溶液中癌胚抗原(CEA)浓度的增大而减小, 并在0.5～20和20～160 ng/mL CEA范围内呈现出良好的线性关系, 检测下限为0.2 ng/mL. 该免疫传感器具有制作简单、重现性好、线性范围宽等优点, 可用于临床上对CEA的检测.

关键词: 免疫传感器, 聚2,6-二氨基吡啶(pPA), 硫堇(Thi), 纳米金(nano-Au), 癌胚抗原(CEA)

The poly 2,6-pyridinediamine (pPA) film was formed on the glassy carbon electrode by utilizing electropolymerization, which yielded an interface with amine groups for the assembly of thionine (Thi) and colloidal Au. The electrochemical characteristics of the immunosensor were studied by cyclic voltammetry. The factors influencing the performance of the resulting biosensor were studied in detail. Under the optimized conditions, the current response of the proposed immunosensor decreased linearly in two concentration ranges of CEA from 0.5 to 10 ng/mL and from 10 to 120 ng/mL with a detection limit of 0.2 ng/mL. Moreover, the studied immunosensor exhibited simple fabrication, good reproducibility and a broad linear range, which made it potentially application for determination of CEA in clinical analysis.

Key words: immunosensor, poly(2,6-pyridinediamine), thionine, gold nanoparticle, carcinoembryonic antigen