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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (23): 2865-2869.DOI: 10.6023/A1012104 上一篇    下一篇

研究论文

基于碳纳米管和离子液体复合物修饰电极的免疫传感器检测莱克多巴胺

陈昌云*,1, 张红琳1, 柳闽生1, 颜妍2, 赵波2   

  1. (1 南京晓庄学院生物化工与环境工程学院 南京 211171)
    (2 南京师范大学化学与材料科学学院 南京 210097)
  • 投稿日期:2010-12-10 修回日期:2011-02-10 发布日期:2011-08-15
  • 通讯作者: 陈昌云 E-mail:yhjiangccy@126.com
  • 基金资助:

    江苏省教育厅高新技术产业化项目;南京大学生命分析化学教育部重点实验室开放课题资助

An Electrochemical Immunosensor for the Determination of Ractopamine Based on Multi-walled Carbon Nanotubes and Ionic Liquid Composite

Chen Changyun*,1; Zhang Honglin1; Liu Minsheng1; Yan Yan2; Zhao Bo2   

  1. (1 School of Biochemical & Environmental Engineering, Nanjing Xiaozhuang University, Nanjing 211171)
    (2 College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097)
  • Received:2010-12-10 Revised:2011-02-10 Published:2011-08-15

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1352

被引次数

39

建立了一种新的电化学免疫传感器方法, 将多壁碳纳米管(MWCNTs)和室温离子液体1-丁基-3-甲基咪唑四氟硼酸盐([BMIM]BF4)复合物, 和偶联了牛血清蛋白(BSA)的莱克多巴胺抗原, 使用Nafion固定在电极上, 利用莱克多巴胺抗体和抗原之间特定反应的竞争模式, 以K3Fe(CN)6为探针, 通过循环伏安法和差分脉冲伏安法监测免疫反应, 对溶液中莱克多巴胺的浓度进行检测. 线性范围宽(1～1500 ng/mL), 检测限可低至0.3 ng/mL. 同时, 我们对猪饲料实际样品进行测定, 回收率令人满意.

关键词: 多壁碳纳米管, 离子液体, 莱克多巴胺, 电化学免疫传感器

We have developed a new method of electrochemical immunosensor for the determination of ractopamine (RAC). The modified electrodes were prepared as follows. First, the multi-walled carbon nanotubes and ionic liquid composites were cast on glassy carbon electrode (GCE), and then the ractopamine-bovine serum albumin antigens were cast on GCE. At last, we dipped a few Nafion onto the electrode. Cyclic voltammetry and differential pulse voltammetry were employed to monitor the fabrication steps of immunoreaction system in solutions containing K3Fe(CN)6 as redox probes. The immunosensor demonstrates excellent RAC detection features with a broad linear range of 1 to 1500 ng/mL and a detection limit of 0.3 ng/mL. The tests with spiked animal feed samples showed excellent recovery for ractopamine.

Key words: multi-walled carbon nanotube, ionic liquid, ractopamine, electrochemical immunosensor