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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (22): 2619-2623. 上一篇    下一篇

研究论文

乙二醇-磷霉素改性氧化锆色谱固定相的制备及其在蛋白质分离中的应用

张淑琼*,a,b，邹凤平c，李 烃b   

  1. (a长江师范学院化学及环境科学系 涪陵 408100) (b武汉大学化学与分子科学学院 武汉 430072) (c中国地质大学材料科学与化学工程学院 武汉 430074)
  • 投稿日期:2008-10-24 修回日期:2009-05-10 发布日期:2009-07-23
  • 通讯作者: 张淑琼 E-mail:hezhangshuqiong@163.com
  • 基金资助:

    教育部“新世纪优秀人才支持计划”基金（NCET-05-0616）;国家杰出青年科学基金（no.20625516）

Preparation of Glycol-Fosfomycin Modified Zirconia Chromatography Stationary Phases and Its Application to the Separation of Proteins

Zhang, Shuqiong*,a,b，Zou, Fengpingc，Li, Tingb   

  1. (a Department of Chemistry and Environment Science, Changjiang Teachers College, Chongqing 408100) (b College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072) (c Material Science and Chemical Engineering, China University of Geosciences, Wuhan 430074)
  • Received:2008-10-24 Revised:2009-05-10 Published:2009-07-23

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被引次数

2

合成了分离蛋白质的乙二醇-磷霉素钠改性氧化锆高效液相色谱固定相, 通过漫反射红外光谱、元素分析等分析方法对该固定相进行了表征. 以溶菌酶、核糖核酸酶A、细胞色素C和糜蛋白酶四种标准碱性蛋白质为探针, 系统地考察了固定相的疏水相互作用色谱性能. 结果表明, 乙二醇-磷霉素改性氧化锆固定相对蛋白质有一定的保留, 表现出较高的分离选择性.

关键词: 蛋白质, 乙二醇, 磷霉素钠, 氧化锆, 疏水相互作用色谱

A novel glycol-fosfomycin modified zirconia (G-F-ZrO2) stationary phase was prepared and characterized by elemental analysis, FT-IR and N2 adsorption. Its chromatographic performance was evaluated using four standard proteins including lysozyme (Lys), ribonuclease-A (RNase-A), a-chymotrypsin (α-Chy) and Cytochrome C (Cyt-C) as probes, and the effects of salt concentration, pH, salt type in eluents and temperature on retention behavior of proteins were investigated. The results indicate that the stationary phase behaves mainly as a hydrophobic interaction chromatographic packing with salt concentration in the mobile phase above 1.0 mol•L－1. At the same time, the retention mechanism between protein and G-F-ZrO2 stationary phase was also discussed.

Key words: protein, glycol, fosfomycin, ZrO2, hydrophobic interaction chromatography