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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (8): 781-785. 上一篇    下一篇

研究论文

血红蛋白在纳米孔径氧化铝膜修饰电极上的直接电化学

武五爱 郭满栋* 尹志芬 尉景瑞
李婧芳 李 君 张 宇

  

  1. (山西师范大学化学系 临汾 041004)

  • 投稿日期:2008-08-23 修回日期:2008-10-28 发布日期:2009-04-28
  • 通讯作者: 郭满栋

Direct Electrochemistry of Hemoglobin Immobilized on Glassy Carbon Electrode Modified with Anodic Alumina Oxide Films

Wu, Wuai Guo, Mandong* Yin, Zhifen Wei, Jingrui
Li, Jingfang Li, Jun Zhang, Yu
  

  1. (Department of Chemistry, Shanxi Normal University, Linfen 041004)
  • Received:2008-08-23 Revised:2008-10-28 Published:2009-04-28
  • Contact: Guo, Mandong

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1836

被引次数

21 | 1

运用电化学包埋法成功将血红蛋白(Hb)固定于纳米孔阳极氧化铝膜(AAO)修饰的玻碳电极(GC)表面, 制得Hb/PPy/AAO/GC修饰电极, 并对Hb/PPy/AAO/GC修饰电极的制备条件进行了优化. 研究了Hb/PPy/AAO/GC修饰电极在磷酸缓冲液(pH＝6.8)中的电化学行为, 探讨了血红蛋白在AAO修饰电极表面的直接电子转移机理. 结果表明阳极氧化铝膜不仅保持了血红蛋白的生物活性, 而且通过它的纳米尺寸效应, 实现了Hb与电极之间的直接电子转移. 其研究内容对生命科学和临床医学有着重要意义.

关键词: 血红蛋白, 氧化铝膜, 化学修饰电极

A steady Hb/PPy/AAO/GC electrode was obtained by successfully immobilizing hemoglobin (Hb) on a glassy carbon (GC) electrode, which was modified using anodic alumina oxide (AAO) films. Its optimum experimental conditions were found. The electrochemistry behavior of the Hb/PPy/AAO/GC biosensor in PBS (pH＝6.8) and the direct electron transfer mechanism of Hb were discussed, finding that the anodic alumina oxide films kept the biological activity of Hb. Moreover through nano-size effect of the films, the direct electron transfer between Hb and the electrode finished. The study is important to life science and clinical medicine.

Key words: hemoglobin, anodic alumina oxide films, chemical modified electrode