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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (18): 1925-1929. 上一篇    下一篇

研究论文

二硫键修饰的介孔二氧化硅微球的制备与表征

唐倩倩，袁丽，杨东*，胡建华   

  1. (复旦大学高分子科学系 聚合物分子工程教育部重点实验室 先进材料实验室 上海 200433)
  • 投稿日期:2010-04-27 修回日期:2010-05-24 发布日期:2010-06-12
  • 通讯作者: 胡建华 E-mail:hujh@fudan.edu.cn

Preparation and Characterization of Disulfide Bond Functionalized Mesoporous Silica Microspheres

TANG Qian-Qian, YUAN Li, YANG Dong, HU Jian-Hua   

  1. (Department of Macromolecular Science, Key Laboratory of Molecular Engineering of Polymers Ministry of Education, Laboratory of Advanced Materials, Fudan University, Shanghai 200433)
  • Received:2010-04-27 Revised:2010-05-24 Published:2010-06-12

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3079

被引次数

14 | 2

以一步法合成了巯丙基功能化的介孔二氧化硅微球, 微球的粒径在130～150 nm左右, 平均孔径为2.79 nm. 以所合成的微球为载体, 在其内、外表面修饰二硫键后, 在二硫键的另一端接枝了异硫氰酸荧光素(FITC), 并以FITC为标记物研究了二硫键在谷胱甘肽溶液中的断键情况. 实验结果表明, 微球上的二硫键具有在磷酸盐缓冲溶液(PBS, pH＝7.4)中稳定存在, 而在一定浓度的谷胱甘肽溶液中却能发生断键反应. 以高分辨透射电镜(HR-TEM)、N2吸附-脱附实验表征了介孔二氧化硅微球的表面及孔道情况, 用Zeta电位分析、元素分析、热失重分析(TGA)等手段表征了微球上二硫键的接枝情况, 用荧光光度计(FLS)及紫外-可见分光光度计(UV-Vis)研究了谷胱甘肽对二硫键的断键情况的影响.

关键词: 介孔二氧化硅微球, 二硫键, 异硫氰酸荧光素(FITC), 谷胱甘肽

Mercapto-propyl functionalized mesoporous silica microspheres (MSN-SH) were synthesized by one-step method. The size of the microspheres was ranged from 130 to 150 nm, and the average pore size was about 2.79 nm. After modified the outer and inner surfaces of the MSN-SH with disulfide bonds, the other end of the disulfide bond was labeled with fluorescein isothicyanate (FITC). The stability of the disulfide bond functionalized MSN was studied in glutathione solvent. The results showed that the disulfide bond would be broken in glutathione solvent, while it could keep stable in the phosphate buffer (PBS, pH＝7.4). High resolution transmission electron microscopy (HR-TEM) and N2 adsorption-desorption isotherm were utilized to study the morphology and porous of the MSN, and elemental analysis, thermal gravimetric analysis (TGA), Zeta potential analysis methods were used to characterize the amount of the disulfide bond grafted on the MSN. The stability of the disulfide bond in glutathione solvent was also studied by fluorescence spectrometer (FLS) and UV-visible spectrophotometer (UV-Vis).

Key words: mesoporous silica microspheres, disulfide bond, fluorescein isothicyanate (FITC), glutathione