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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (01): 101-105. 上一篇    下一篇

研究论文

应用傅里叶变换红外光谱区分鉴定四种食源性致病菌的研究

路春霞，刘源，孙晓红，潘迎捷，赵勇*   

  1. (上海海洋大学食品学院 上海 201306)
  • 投稿日期:2010-04-14 修回日期:2010-06-07 发布日期:2010-08-16
  • 通讯作者: 路春霞 E-mail:zilxyu@163.com
  • 基金资助:

    “十一五”国家科技支撑计划课题—世博科技专项;国家自然科学基金项目;上海市青年科技启明星计划资助项目;上海市教育委员会重点学科建设项目资助

Discrimination and Identification of Four Foodborne Bacteria Using Fourier Transform-Infrared Spectroscopy

LU Chun-Xia, LIU Yuan, SUN Xiao-Hong, PAN Ying-Jie, ZHAO Yong   

  1. (1College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306)
  • Received:2010-04-14 Revised:2010-06-07 Published:2010-08-16

PDF

1592

被引次数

38

利用傅里叶变换红外光谱技术(FT-IR)对大肠杆菌O157: H7、肠炎沙门氏菌、单核增生性李斯特菌、金黄色葡萄球菌这四种典型食源性致病菌进行了研究. 对其红外谱图进行基线校正和归一化处理, 对其谱带归属进行判别分析, 选取具有菌株特性的1500～800 cm－1的谱带, 进行一阶导数运算, 结合化学计量学方法主成分分析(PCA)和聚类分析(CA), 并进行相关系数的统计学分析, 得出以下结果: 四种食源性致病菌最大相关系数为0.937, 最小相关系数为0.058, 而且四种不同的细菌在PCA和CA分析图上也达到了很好的区分效果. 研究表明, FT-IR有望成为一种快速检测食源性致病菌的新方法.

关键词: 食源性致病菌, FT-IR, 区分, 鉴定

Fourier transform-infrared spectroscopy (FT-IR) is used to discriminate and identify four food- borne bacteria Escherichia coli O157:H7, Salmonella enteritidis, Staphylococcus aureus and Listeria mono- cytogenes. The representative infrared spectra were adjusted by automatic baseline correction and normalize scale. The spectra are then analysed through first derivative transformation between 800 cm－1 and 1500 cm－1 in order to compare the similarities and differences between spectra of the four chosen bacteria. Results show that the minimum correlation coefficients between the bacteria is 0.058, while the maximum is 0.937. The results also showed significant differences between different foodborne bacteria. Moreover, principal component analysis (PCA) and cluster analysis (CA) revealed clear segregations between different bacterial strains. It is concluded that FT-IR techniques have great potential as routine methods in Foodborne bacteria.

Key words: foodborne bacteria, FT-IR, distinction, identification