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化学学报
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化学学报 ›› 1994, Vol. 52 ›› Issue (5): 496-499. 上一篇    下一篇

研究论文

色氨酸-亚硝酸体系荧光性质的研究及应用

揭念琴;杨景和;孟凡琴;黄慕宾   

  1. 山东大学化学系.济南(250100)
  • 发布日期:1994-05-15

Fluorescence properties of tryptophan-nitrous acid system and its application

Jie Nianqin;Yang Jinghe;Meng Fanqin;Huang Mubin   

  1. Shandong Univ, Dept Chem.Jian(250100)
  • Published:1994-05-15

PDF

616

被引次数

1

本文首次提出了利用亚硝酸氧化色氨酸的荧光反应测定色氨酸的方法。亚硝酸氧化色氨酸,继而在氢氧化钠介质中生成荧光物质,其激发波长和发射波长分别是:λ~e~x=320nm,λ~e~m=392nm。当色氨酸浓度为0.2～1.6μg/mL时,工作曲线呈良好的线性。本方法用于测定蛋白质样品中的色氨酸,结果令人满意。本文还对反应产物的特性进行了研究。

关键词: 荧光特性, 亚硝酸, 色氨酸, 蛋白质, 荧光分光光度法

Tryptophan is oxidized by nitrous acid at elevated temperature produce a phenolic intermediate which further reacts with nitrous acid to form a fluorescent species. The flluorescent species has the excitation and emission maxima at 320 and 392nm, respectively. The fluorescent intensity is linear over the range of 0.2-1.6μg/mL tryptophan. The method has been applied to the determination of tryptophan in synthetic and protein samples. A plausible mechanism is proposed to explain this behaviour.

Key words: FLUORESCENCE CHARACTERISTIC, NITROUS ACID, TRYPTOPHAN, PROTEIN, FLUOROSPECTROPHOTOMETRY

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