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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (18): 1876-1880. 上一篇    下一篇

研究论文

H2O2-鲁米诺-荧光素钠-K2CO3高灵敏化学发光法测定二氧化碳

范顺利1,2, 屈芳2, 林金明*,1   

  1. (1中国科学院生态环境研究中心 环境化学与生态毒理学国家重点实验室 北京 100085)
    (2河南师范大学化学与环境科学学院 河南省环境污染控制重点实验室 新乡 453007)
  • 投稿日期:2005-12-05 修回日期:2006-03-03 发布日期:2006-09-28
  • 通讯作者: 林金明

Highly Sensitive Determination of Carbon Dioxide with H2O2-Luminol-Uranine-K2CO3 Chemiluminescent Reaction

FAN Shun-Li1,2; QU Fang2; LIN Jin-Ming*,1   

  1. (1 State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085)
    (2 Henan Key Laboratory for Environmental Pollution Control, College of Chemistry and Environmental Sci-ences, Henan Normal University, Xinxiang 453007)
  • Received:2005-12-05 Revised:2006-03-03 Published:2006-09-28
  • Contact: LIN Jin-Ming

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在碱性介质中, CO32－对H2O2氧化鲁米诺化学发光反应具有重要作用, 荧光素钠对该反应具有很强的增敏作用. 据此, 建立了化学发光法测定二氧化碳的新方法. 方法的线性范围为1.0×10－10～5.0×10－6 mol•L－1 CO32－, 检出限为 1.2×10－11 mol•L－1 CO32－ (相当于5.3×10－10 g•L－1 CO2). 该方法用于室内外空气中二氧化碳含量的测定, 相对标准偏差1.8%～2.1% (n＝11), 加标实验回收率97.6%～101.4%. 论文还探讨了反应的发光机理, 发光反应很可能是由溶液中的CO32－与H2O2作用而产生的活性自由基引发, 荧光素钠对发光的增敏作用为化学能量转移过程.

关键词: 化学发光, 鲁米诺, 荧光素钠, 二氧化碳

The chemiluminescent (CL) reaction for the oxidation of luminol with hydrogen peroxide in an alkaline medium was greatly affected by the presence of carbonate. The intensity of CL reaction was evidently enhanced by the addition of uranine. Based on this, a novel CL method for the determination of carbon dioxide was established. The method provided the detection a wide linear concentration rang from 1.0×10－10 to 5.0×10－6 mol•L－1 and a low detection limit of 1.2×10－11 mol•L－1 carbonate or 5.3×10－10 g•L－1 carbon dioxide. It has been applied to determination of carbon dioxide in indoor and outdoor air with relative standard deviations (RSD) of 1.8%～2.1% (n＝11). The recoveries of the spiked samples were 97.6%～101.4%. A reasonable mechanism was also proposed. The CL reaction is probably triggered by the interaction of H2O2-K2CO3 and then enhanced by uranine via chemical energy transfer from luminol to uranine.

Key words: chemiluminescence, luminol, uranine, carbon dioxide