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化学学报
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化学学报 ›› 2002, Vol. 60 ›› Issue (10): 1867-1871. 上一篇    下一篇

研究论文

阿霉素与牛血清白蛋白结合作用的研究

黄波;邹国林;杨天鸣   

  1. 武汉大学生命科学学院,武汉(430072);中南民族大学化学系,武汉(430074)
  • 发布日期:2002-10-15

Studies on the Interaction between Adriamycin and Bovine Serum Albumin

Huang Bo;Zou Guolin;Yang Tianming   

  1. College of Life Sciences, Wuhan University,Wuhan(430072)
  • Published:2002-10-15

PDF

1578

被引次数

419 | 72

结合荧光光谱和吸收光谱研究了阿霉素与牛血清白蛋白间的结合作用，确定了 阿霉素对牛血清白蛋白的荧光猝灭过程的猝灭机理，测定了不同酸度条件下该结合 反应的结合常数、结合位点数，依据能量转移理认确定了药物和蛋白间的结合距离 。通过比较阿霉素和去糖基阿霉素与牛血清白蛋白的相互作用，结合阿霉素对蛋白 构象的影响，讨论了药物与蛋白的结合模式。

关键词: 阿霉素, 牛血清白蛋白, 荧光分光光度法, 结合

The binding reaction between adriamycin (ADR) and bovine serum albumin (BSA) was investigated by absorption spectrometry and fluorescence measurement. It is proved that the fluorescence quenching of BSA by ADR is a result of the formation of ADR-BSA complex. The equilibrium constant K and the number of binding sites n were measured at different pH by fluorescence quenching method. The binding distance r and transfer efficiency E between ADR and BSA were also obtained according to Forster theory of non-radiation energy transfer. The effect of ADR on the conformation of BSA was also analyzed using synchronous fluorescence spectroscopy. By studying the binding of 7- deoxyadriamycinone to BSA, the binding mode between ADR and BSA was discussed.

Key words: adriamycin, BOVINE SERUM ALBUMIN, FLUOROSPECTROPHOTOMETRY, BINDING

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