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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (24): 2705-2711. 上一篇    下一篇

研究论文

配合物[Co2(EGTB)Cl2]•(BF4)2•5H2O与DNA相互作用的研究

朱 莉*,a,b 彭 斌b 凌 友b 林原斌*,a

  

  1. (a湘潭大学化学学院 湘潭 411105)
    (b湖南科技大学化学化工学院 分子构效关系湖南省普通高等学校重点实验室 湘潭 411201)

  • 投稿日期:2008-07-16 修回日期:2008-10-27 发布日期:2008-12-28
  • 通讯作者: 朱莉

Interaction of Complex [Co2(EGTB)Cl2]•(BF4)2•5H2O with DNA

ZHU, Li *,a,b PENG, Bin b LING, You b LIN, Yuan-Bin *,a   

  1. (a Chemistry Institute, Xiangtan University, Xiangtan 411105)
    (b Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China)
  • Received:2008-07-16 Revised:2008-10-27 Published:2008-12-28
  • Contact: ZHU, Li

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用紫外光谱法、荧光光谱法、黏度法、凝胶电泳法研究了双核钴(II)配合物[Co2(EGTB)Cl2]•(BF4)2•5H2O和DNA的相互作用, 在pH＝7.2的缓冲体系中, 求得配合物与DNA的结合常数. 结果表明, 配合物在接近生理条件下能有效地断裂pBR322DNA, 同时可使DNA的粘度增加, 使EB-DNA体系的荧光强度降低. 配合物与DNA的荧光光谱和紫外光谱表明, 配合物与DNA的作用既存在部分插入结合又存在静电结合模式.

关键词: 钴(II)配合物, 合成, DNA, 插入结合, 静电结合, 超螺旋脱氧核糖核酸内切酶(pBR322DNA)

The interaction of Co(II) complex [Co2(EGTB)Cl2]•(BF4)2•5H2O with DNA has been investigated by electronic absorption spectroscopy, fluorescence spectroscopy, viscosity and gel electrophoresis measurements. The apparent binding constants of the complex with DNA was obtained at pH＝7.2. The results indicated that the Co(II) complex could cleave pBR322DNA effectively at physiological pH and temperature, and also increase the viscosity of DNA. The emission intensity was quenched as the complex was added to the EB-DNA system. The results suggest that the interaction of Co(II) complex with DNA belong to both partial intercalation and static electricity attraction mode.

Key words: Co(II) complex, synthesis, DNA, partial intercalation, electricity attraction, pBR322DNA