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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (6): 555-563. 上一篇    下一篇

研究论文

配合物[RE2(H2L)2(HL)2(2,6-H2PDA)(H2O)2]•2H2O的合成、 晶体结构及其与DNA作用方式初探

陈凤英a,b 何水样*,a 刘 峰a 王刘杰a

  

  1. (a西北大学化学系 陕西省物理无机化学重点实验室 西安 710069)
    (b商洛学院化学系 商洛 726000)

  • 投稿日期:2008-05-12 修回日期:2008-11-21 发布日期:2009-03-28
  • 通讯作者: 何水样

Synthesis, Crystal Structure and Preliminary DNA-binding Studies of [RE2(H2L)2(HL)2(2,6-H2PDA)(H2O)2]•2H2O

Chen, Fengying a,b He, Shuiyang *,a Liu, Feng a Wang, Liujie a   

  1. (a Shaanxi Key Laboratory of Physico-inorganic Chemistry, Department of Chemistry, Northwest University, Xi’an 710069)
    (b Department of Chemistry, Shangluo University, Shangluo 726000)

  • Received:2008-05-12 Revised:2008-11-21 Published:2009-03-28
  • Contact: He, Shuiyang

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以N-(2-异丙酸)-邻羟基苯甲酰腙(C10H10N2O4, H3L)、2,6-吡啶二甲酸(2,6-H2PDA)与RE(NO3)3•nH2O (RE＝Pr, Eu)在室温下反应, 合成了配合物1 [Pr2(H2L)2(HL)2(2,6-H2PDA)(H2O)2]•2H2O和配合物2 [Eu2(H2L)2(HL)2(2,6-H2PDA)- (H2O)2]•2H2O, 对其进行了元素分析、红外光谱、紫外光谱等表征, 测定了两种配合物的晶体结构. 通过紫外吸收光谱、荧光发射光谱和稳态荧光猝灭方法及其与溴化乙锭(EB)的竞争实验研究了两种配合物与小牛胸腺DNA的作用情况. 结果表明, 两种配合物与小牛胸腺DNA均是以插入方式结合的.

关键词: 稀土, N-(2-异丙酸)-邻羟基苯甲酰腙, 2,6-吡啶二甲酸, 晶体结构, 小牛胸腺DNA

Complex 1 [Pr2(H2L)2(HL)2(2,6-H2PDA)(H2O)2]•2H2O and complex 2 [Eu2(H2L)2(HL)2(2,6- H2PDA)(H2O)2]•2H2O were obtained by reactions of N-(2-propionic acid)-salicyloyl hydrazone (C10H10N2O4, H3L), 2,6-pyridinedicarboxylic acid (2,6-H2PDA) and RE(NO3)3•nH2O (RE＝Pr, Eu) at room temperature, and characterized by elemental analyses, IR, UV, and single crystal X-ray diffractometry. In addition, the DNA binding properties of the two complexes were studied by means of UV absorption spectrum, emission spectroscopic spectrum, steady-state emission quenching and DNA competitive binding with ethidium bromide (EB). The results indicated that the two complexes bound to calf-thymus DNA in an intercalative mode.

Key words: rare earth, N-(2-propionic acid)-salicyloyl hydrazone, 2,6-pyridinedicarboxylic acid, crystal structure, calf-thymus DNA