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化学学报
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化学学报 ›› 2003, Vol. 61 ›› Issue (12): 2029-2032. 上一篇    下一篇

研究论文

三核铜配合物[Cu_3L_2](ClO_4)_2(H_2L=1,3-双（2-羟基-1-苯基-亚甲基）-丙烷)的合成与对pBR322DNA的切断作用

沈鹤柏;杨永桃;杨仕平;蒯丽衡;姜霁;陈红梅   

  1. 上海师范大学生命与环境科学学院
  • 发布日期:2003-12-15

Synthesis of Trinuclear Copper (II) Complex [Cu_3L_2] (ClO_4)_2 and Its Cleavage of pBR332 DNA

Shen Hebai;Yang Yontao;Yang Shiping;Kuai Liheng;Jiang Ji;Chen Hongmei   

  1. College of Life and Environment Science, Shanghai Normal University
  • Published:2003-12-15

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利用席夫碱配体[H_2L = 1，3-双（2-羟基-1-苯基-亚甲基）-丙烷]与铜盐作 用，得到了配合物[Cu_3L_2](ClO_4)_2，并用X射线单晶衍射测定了其晶体结构。 研究了该三核铜配合物与质粒pBR322 DNA之间的相互作用，琼脂糖凝胶电泳实验结 果表明该配合物能够将pBR322 DNA从超螺旋构型切割成开环型和线型。同时，紫外 光谱实验可以推测，配合物在对pBR322 DNA进行切断时，它们之间在一种插入作用 。

关键词: 铜络合物, 席夫碱, X射线衍射分析, 晶体结构, 紫外分光光度法

A trinudear copper (II) complex with Schiff-base ligand, [Cu_3L_2] (ClO_4)_2, [ H_2L = 1,3-bis(2-hydroxylbenzoylimino)propane], was synthesized and characterized by X-ray crystal diffraction analysis. The interaction of the complex with pBR322 DNA has been investigated by agaiose gel electrophoresis. The complex has been found to promote cleavage of pBR322 DNA from supercoiled form I to the open circular form II and to linearized form III , showing that the complex has high cleaving efficiency to DNA. The UV spectroscopy demonstrates that the complex can bind to DNA by partial intercalation.

Key words: COPPER COMPLEX, SCHIFF BASE, XRD, CRYSTAL STRUCTURE, UV

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