关键词: 氨基酸, 质谱法, 磷酰化, 二异丙基亚磷酸酯, 磷酰氨基酸

The pos. and neg. FAB-MS of the mixed twenty phosphorylated amino acids are reported. The triethylamine and glycerol matrix background was suppressed. By this in situ phosphorylation method, all twenty amino acids could be detected no matter how much phosphorylation reagent was used. The basic amino acids showed much greater response to the pos. FAB-MS. While the neutral and acidic amino acids were give much more intense mol. ion peaks in the neg. FAB-MS. This method could apply to the qual. detection of the amino acids in samples.

Key words: AMINO ACID, MASS SPECTROGRAPHY, PHOSPHORYLATION