关键词: 紫外分光光度法, 反应动力学, 黄酮类化合物, 蒽醌 P, 牛, 荧光分光光度法, 大黄素, 血清白蛋白, 结合反应

Stopped flow studies how that the binding equilibrium of emodin to bovine serum albumin (BSA) is established within 100 ms. The adsorption max. of these compounds exhibit red shift upon binding and the absorbance enhanced. The fluorescence quenching associated with the binding of these compounds to BSA was used to measure binding constants, and the distance of Trp-212 to the first binding site were calculated, on the basis of the energy transfer between BSA and bound complexes. An allosteric domain model of emodin-BSA complex was postulated.

Key words: ULTRAVIOLET SPECTROPHOTOMETRY, REACTION KINETICS, FLAVONOID, ANTHRAQUINONE P, CATTLE, FLUOROSPECTROPHOTOMETRY, EMODIN, SERUM ALBUMIN