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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (22): 2597-2603. 上一篇    下一篇

研究论文

水溶性桥联双卟啉与DNA相互作用的研究

王凯，张智，郭茜妮，鲍小平，李早英*   

  1. (武汉大学化学与分子科学学院 武汉 430072)
  • 投稿日期:2007-01-25 修回日期:2007-05-20 发布日期:2007-11-28
  • 通讯作者: 李早英

Studies on Interaction of Water-Soluble Bridged Porphyrin with DNA

WANG Kai; ZHANG Zhi; GUO Qian-Ni; BAO Xiao-Ping; LI Zao-Ying*   

  1. (College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072)
  • Received:2007-01-25 Revised:2007-05-20 Published:2007-11-28
  • Contact: LI Zao-Ying

PDF

890

被引次数

49

以4,4'-二羧酸-2,2'-联吡啶为桥联试剂, 合成了一种含8个阳离子的水溶性桥联双卟啉(PD). 以5,10,15,20-四(4-N-甲基吡啶盐)卟啉(H2TMPyP)为参照物, 使用紫外-可见光谱、荧光光谱、圆二色谱研究了水溶性双卟啉与小牛胸腺DNA (CT DNA)的相互作用, 以溴化乙啶(EB)竞争法测定了PD与CT DNA的表观键合常数(Kapp)为1.2×106 L•mol－1 (H2TMPyP为6.9×106 L•mol－1), 并使用凝胶电泳研究了PD对pBR322质粒DNA的切割能力. 实验结果表明PD与CT DNA的作用方式是插入和外部结合的混合模式.

关键词: 双卟啉, DNA, 表观键合常数, 光切割

A water-soluble porphyrin dimer (PD) containing eight positive charges, bridged by 4,4'-dicarboxy-2,2'-bipyridyl, has been synthesized. Taking meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (H2TMPyP) as a reference compound, interaction of the water-soluble porphyrin dimer with DNA was investigated by using absorption, fluorescence, CD spectroscopy, and the apparent affinity binding constant (Kapp) of PD binding to CT DNA was measured to be 1.2×106 L•mol－1 by a competition method with ethidium bromide (EB) (that of H2TMPyP was 6.9×106 L•mol－1). The cleavage ability of PD to pBR322 plasmid DNA was studied by gel electrophoresis. The results suggest that the binding modes of PD and CT DNA involve in both of intercalation and outside binding.

Key words: porphyrin dimer, DNA, apparent affinity binding constant, photocleavage