化学学报 ›› 2007, Vol. 65 ›› Issue (24): 2935-2940. 上一篇    下一篇

研究论文

丝素蛋白修饰改性的聚羟基脂肪酸酯支架上人体平滑肌细胞的生长

张翼1, 周平*,1, 潘銮凤*,2, 谢尚喆2, 孙敏1, 李文婷1   

  1. (1教育部聚合物分子工程重点实验室 复旦大学高分子科学系 上海 200433)
    (2复旦大学上海医学院分子生物学实验室 上海 200032)
  • 投稿日期:2007-05-14 修回日期:2007-08-15 发布日期:2007-12-28
  • 通讯作者: 潘銮凤

Growth of Human Smooth Muscle Cells on the Silk Fibroin Modified-Polyhydroxyalkanoate Scaffold

ZHANG Yi1; ZHOU Ping*,1; PAN Luan-Feng*,2; XIE Shang-Zhe2; SUN Min1; LI Wen-Ting1   

  1. (1 Key Laboratory of Molecular Engineering of Polymers, Education Ministry, Department of Macromolecular Science, Fudan University, Shanghai 200433)
    (2 Laboratory of Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032)
  • Received:2007-05-14 Revised:2007-08-15 Published:2007-12-28
  • Contact: PAN Luan-Feng

聚三羟基丁酸脂和聚三羟基己酸脂的共聚物(PHBHHx)是一种具有良好强度和韧性的生物可降解高分子材料, 可作为组织工程心脏瓣膜支架的选择材料之一. 但其生物相容性尚不甚理想. 为此, 本工作利用丝素蛋白修饰改性高分子多孔支架, 以提高支架的生物相容性. 并将人体平滑肌细胞接种在该复合支架上进行体外培养, 以证实改性效果. 其中, 用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)方法测试细胞生长, 评估复合支架的细胞相容性. 并用扫描电子显微镜观察细胞在支架上的生长形态. 结果显示, 丝素蛋白修饰改性后的复合支架更有利于细胞的粘附与生长, 平滑肌细胞在支架上表现出良好的生长形态. 这表明, 丝素能够改善多孔支架的生物相容性, 使PHBHHx/丝素蛋白复合物能更适宜作为组织工程心脏瓣膜的支架材料. 结果对于进一步研究细胞外间质在复合支架上的生长以及体外培养的组织重建有重要的参考意义.

关键词: 组织工程心脏瓣膜, 多孔支架, 丝素蛋白, 聚羟基脂肪酸脂, 人体平滑肌细胞

In order to find an appropriate scaffold for the tissue engineered heart valve, the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), a biodegradable material with a good stress-strain mechanical property, was utilized as a porous scaffold material. But its biocompatibility is not good enough. To improve its biocompatibility, the silk fibroin (SF) was applied to modify the PHBHHx scaffold, and the human smooth muscle cells were seeded on the scaffold in vitro for the demonstration. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was used to test the cell growths and to evaluate the biocompatibility of the scaffold. SEM was used to observe the cell morphologies. The results reveal that the human smooth muscle cells grow better on the silk fibroin modified hybrid scaffold. It proves that the silk fibroin modification can improve the biocompatibility of the PHBHHx scaffold. The silk fibroin modified hybrid PHBHHx scaffold can be potentially used for the heart valve tissue engineering for further research.

Key words: heart valve tissue engineering, porous scaffold, silk fibroin, polyhydroxyalkanoate, human smooth muscle cell