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化学学报
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化学学报 ›› 1983, Vol. 41 ›› Issue (12): 1164-1178. 上一篇    下一篇

研究论文

酵母丙氨酸转移核糖核酸3'-端十九核苷酸(58-76)的合成

陈海室;徐静范;鲍建绍;庄建华;郭荣汉;施才能;陈法贤;张恭勤;冯晓黎;丁晓华;江鹏   

  1. 中国科学院上海有机化学研究所;海军军医学校生化教研室
  • 发布日期:1983-12-15

Synthesis of the 3'-terminal nonadecanucleotide (58-76) of yeast alanine tRNA^+

CHEN HAISHI;XU JINGFAN;BAO JIANSHAO;ZHUANG JIANHUA;GUO RONG HAN;SHI CAINENG;CHEN FAXIAN;ZHANG GONGQIN;FENG XIAOLI;DING XIAOHUA;JIANG PENG   

  • Published:1983-12-15

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428

关键词: 丙氨酸, 转移核糖核酸, 核苷酸

Two new 3'-phosphorylation procedures of oligoribonucleotide have been worked out. By means of using donor with a 3'-terminal phosphate group, under low ratio of donor/acceptor (1:1.1 to 1:2), nonadecanucleotide AUUCCGGACUCGUCCACCAp, which corresponds to the 3'-terminal nucleotides 58-76 of yeast alanine RRNA, has been synthesized in good yields (87-90%) through ligation by T4RNA ligase of four component fragments: AUUC, CGGA, CUCGUCCA and CCAp.

Key words: ALANINE, TRANSFER RIBONUCLEIC ACID, NUCLEOTIDES

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