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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (3): 239-245. 上一篇    下一篇

研究论文

脱氧胆酸钠与支撑磷脂双层膜作用的电化学研究

王建国*, 江黎丽, 惠飞, 杨薇薇, 吕慧, 孙巧花   

  1. (辽宁大学化学科学与工程学院 沈阳 110036)
  • 投稿日期:2006-06-19 修回日期:2006-09-04 发布日期:2007-02-14
  • 通讯作者: 王建国

Study on the Interaction of Sodium Deoxycholate with Supported Bilayer Lipid Membrane by Electrochemistry Method

WANG Jian-Guo*; JIANG Li-Li; HUI Fei; YANG Wei-Wei; LÜ Hui; SUN Qiao-Hua   

  1. (Institute of Chemical Sciences and Engineering, Liaoning University, Shenyang 110036)
  • Received:2006-06-19 Revised:2006-09-04 Published:2007-02-14
  • Contact: WANG Jian-Guo

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1705

被引次数

20 | 11

以支撑磷脂双层膜(supported bilayer lipid membrane, s-BLM)作为生物膜模型, 采用循环伏安法和交流阻抗技术研究了脱氧胆酸钠(sodium deoxycholate, NaDC)与s-BLM的相互作用. 结果表明, NaDC能降低磷脂分子的有序性, 诱发s-BLM上形成孔洞或缺陷, 并且它们之间的这种相互作用对作用时间、NaDC溶液的浓度和pH值以及胆固醇的存在与否具有依赖性, 并且作用后的s-BLM在0.1 mol/L的KCl溶液中能够自我修复, 这表明NaDC与s-BLM的相互作用是可逆的.

关键词: 支撑磷脂双层膜, 循环伏安, 交流阻抗, 脱氧胆酸钠

In this paper, supported bilayer lipid membrane (s-BLM) was served as a biomembrane model. The interaction between sodium deoxycholate (NaDC) and s-BLM was investigated by means of cyclic voltammetry and ac impedance spectroscopy. The results show that NaDC can decrease the orderliness of lecithin molecule and induce pores or defects on s-BLM. The interaction between them depends on time, concentration, pH and cholesterol. The defective s-BLM after interaction can be self-repaired in 0.1 mol/L KCl solution, which indicates that the interaction is reversible.

Key words: supported bilayer lipid membrane, cyclic voltammetry, ac impedance spectroscopy, sodium deoxycholate