SIOC English
化学学报
高级检索

化学学报 ›› 2006, Vol. 64 ›› Issue (14): 1451-1455. 上一篇    下一篇

研究论文

硫杂蒽酮类稀土配合物的合成、表征及与DNA的作用(I)

宋玉民*,1，芦小林1,2，唐慧安1,2，秦淑琪1   

  1. (1西北师范大学化学化工学院 兰州 730070)
    (2天水师范学院生命科学与化学学院 天水 741012)
  • 投稿日期:2005-10-08 修回日期:2006-03-27 发布日期:2006-07-28
  • 通讯作者: 宋玉民

Synthesis, Characterization of the Complex of Thiaxanthone with Rare Earth and the Interactions between the Complex and DNA (I)

SONG Yu-Min*,1, LU Xiao-Lin1,2, TANG Hui-An1,2, QIN Shu-Qi1   

  1. (1 College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070)
    (2 College of Bioscience and Chemistry, Tianshui Normal University, Tianshui 741012)
  • Received:2005-10-08 Revised:2006-03-27 Published:2006-07-28
  • Contact: SONG Yu-Min

PDF

861

被引次数

26 | 4

合成了新的O-(硫杂蒽酮-[2]-基)-氧乙酸及其稀土配合物. 通过元素分析, IR, 1H NMR, UV, DTA-TG和13C NMR谱对其结构进行了表征. 研究表明: 配体羧羰基脱质子后与金属离子配位, 2位氧原子也与金属离子配位, 配合物中含有一定量的配位水, 配合物为非电解质类型. 同时, 研究了O-(硫杂蒽酮-[2]-基)-氧乙酸稀土配合物对质粒DNA的切割作用. 结果表明: 铕的配合物对DNA的切割较明显, 且当配合物浓度增加时, 质粒DNA的超螺旋构型逐渐减少, 而缺刻、开环型构型逐渐增多. 在相同条件下, Eu(III)离子对质粒pBR322DNA几乎没有切割作用; 配体O-(硫杂蒽酮-[2]-基)-氧乙酸对质粒pBR322DNA也有切割作用, 但配合物EuL3对质粒pBR322DNA的切割作用明显强于配体, 表明稀土离子Eu(III)与配体生成配合物后有较好的协同切割作用.

关键词: 合成, 表征, O-(硫杂蒽酮-[2]-基)-氧乙酸, 稀土配合物, 切割DNA

The complexes of thiaxanthon-2-yl-oxyacetate with rare earths were synthesized. The structure of such complexes was characterized by elemental analysis, IR, 1H NMR, UV, DTA-TG and 13C NMR spectrum. The results suggested that their composition should be M∶L＝1∶3 (M＝RE3＋, L＝thiaxanthon-2-yl-oxyacetate), the metal should be coordinated to not only carboxylic oxygen but also ether oxygen, and the complex should be non-electrolyte with coordinating water molecule. The interaction between the Eu(III) complex of thiaxanthon-2-yl-oxyacetate and plasmid DNA was studied by agarose gel electrophoresis. The data showed that the cleavage of plasmid DNA might depend on the concentration of the complex. The higher concentration of the complex caused the larger decrease of DNA super helix. Under the same concentration, the complex and thiaxanthon-2-yl-oxyacetate had obvious plasmid DNA cleavage ability but Eu(III) had very little. The complex had stronger plasmid DNA cleavage ability than ligand. The results suggested that rare earth ions had good synergism with ligand in cleavage of plasmid DNA.

Key words: synthesis, characterization, thiaxanthon-2-yl-oxyacetate, rare earth complex, cleavage of DNA