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化学学报
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化学学报 ›› 2000, Vol. 58 ›› Issue (8): 1031-1036. 上一篇    下一篇

研究论文

β-CD-Shiff碱铜(Ⅱ)配合物模拟酶催化光度法的研究

唐波;刘阳;梁芳珍;陈德展;孙天麟   

  1. 山东师范大学化学系.济南(250014)
  • 发布日期:2000-08-15

Studies on the catalytic spectrophotometry using β-CD-Shiff Cu(Ⅱ) complex as minetic enzyme

Tang Bo;Liu Yang;Liang Fangzhen;Chen Dezhan;Sun Tianlin   

  1. Shandong Normal Univ, Dept Chem.Jinan(250014)
  • Published:2000-08-15

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410

被引次数

63 | 6

合成了Shiff碱2-羟基-1-萘醛缩-2-氨基噻唑(HNATS)及其铜配合物，发现配合物Cu(Ⅱ)-(HNATS)~2具有显著的过氧化物模拟酶活性，可催化H~2O~2氧化4-氨基安替比林与2,4-二氯苯酚偶联反应。研究了β-环糊精对模拟酶催化活性的影响，探讨了反应机理，考察了反应条件和共存物质的影响，建立了新的测定超氧阴离子自由基(O~2^-^.)的分光光度法，线性范围为0～5.0×10^-^4mol/L，检出限为5.0×10^-^6mol/L。方法用于测定人体血液中超氧化物歧化酶(SOD)活性，结果满意。

关键词: 席夫碱, 铜络合物, 环糊精, 超氧化物歧化酶, 模拟酶, 分光光度法, 噻唑P, 过氧化氢, 安替比林, 苯酚P

A new Schiff base, 2-hydroxy-1-napthaldehyde-2-aminothiazole (HNATS) and its Cu(Ⅱ) complex were synthesized. The complex [Cu(Ⅱ)-(HNATS) ~2] exhibits efficient peroxidase-like catalytic activity, and catalyzes the coupling reaction of 2,4-dichlorophenol and 4-amino antipyrine in the presence of hydrogen peroxide. The effect of β-CD on the catalytic activity of the mimetic enzyme was studied. The reaction mechanism, optimal reaction conditions and effects of coexisting substances were also investigated. Based on the reaction, the determination of (O~2^-^.) by this new spectrophotometric method was developed with a linear range of 0～5.0×10^-^4 mol/L and detection limit of 5.0×10^-^6 mol/L. The proposed method was used to determine superoxide dismutase (SOD) activity with satisfactory results.

Key words: SCHIFF BASE, COPPER COMPLEX, CYCLODEXTRIN, SUPEROXIDE DISMUTASE, MODELS OF ENZYME, SPECTROPHOTOMETRY, THIAZOLE P, HYDROGEN PEROXIDE, ANTIPYRINU, PHENOL P

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