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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (15): 1796-1802. 上一篇    下一篇

研究论文

基于壳聚糖-纳米金/纳米普鲁士蓝/L-半胱氨酸修饰的 葡萄糖传感器的研究

(西南大学化学化工学院重庆市现代分析化学重点实验室 重庆 400715)

  

  1. 在金电极表面修饰带正电荷的L-半胱氨酸, 再利用静电吸附作用固定纳米普鲁士蓝(nano-PB), 然后利用壳聚糖-纳米金复合膜将葡萄糖氧化酶(GOD)固定于修饰电极表面, 制成新型的葡萄糖传感器. 通过交流阻抗技术, 循环伏安法和计时电流法考察了电极的电化学特性. 在优化的实验条件下, 该传感器在葡萄糖浓度为3.0×10－6～1.0×10－3 mol/L范围内有线性响应, 检测下限为1.6×10－6 mol/L. 此外该传感器具有响应快、稳定性好和选择性良好的特点, 能有效排除常见干扰物质如抗坏血酸、尿酸等对测定的影响.

  • 投稿日期:2007-11-28 修回日期:2008-01-29 发布日期:2008-08-14
  • 通讯作者: 袁若

Glucose Biosensor Based on Chitosan-nano Gold/nano-Prussian Blue/L-cysteine Modified Gold Electrode

FU, Ping YUAN, Ruo* CHAI, Ya-Qin YIN, Bing CAO, Shu-Rui
CHEN, Shi-Hong LI, Wan-Yang
  

  1. (Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering,
    Southwest University, Chongqing 400715)
  • Received:2007-11-28 Revised:2008-01-29 Published:2008-08-14
  • Contact: YUAN, Ruo

PDF

2369

被引次数

66 | 12

在金电极表面修饰一层L-半胱氨酸，再利用静电吸附作用固定纳米普鲁士蓝(nano-PB)，然后利用壳聚糖-纳米金复合膜将葡萄糖氧化酶(GOD)固定于修饰电极表面，制成新型的葡萄糖传感器.通过交流阻抗技术，循环伏安法和计时电流法考察了电极的电化学特性.在优化的实验条件下，该传感器在葡萄糖浓度为3.0×10-6～1.0×10-3 mol/L范围内有线性响应，检测下限为1.6×10-6 mol/L.此外该传感器具有响应快、稳定性好和选择性良好的特点，能有效排除常见干扰物质如抗坏血酸、尿酸等对测定的影响.

关键词: L-半胱氨酸, 纳米普鲁士蓝, 葡萄糖传感器, 纳米金, 壳聚糖

A novel glucose biosensor has been presented in this paper. First, L-cysteine was immobilized onto a gold electrode to form a positively charged surface, then nano-Prussian blue was assembled on the modified surface as an electron transfer mediator. Finally, the hybrid film of chitosan-nano gold and glucose oxidase (GOD) was immobilized on the electrode to fabricate the proposed biosensor. The modifying process was characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and transmission electron microscopy (TEM). And the effects of applied potential and pH on the response currents were also investigated. Under optimal conditions, the resulting biosensor displayed a rapid response to glucose and the linear range of the biosensor was from 3.0×10－6 to 1.0×10－3 mol/L with a detection limit of 1.6×10－6 mol/L (S/N＝3). Moreover, the interferents influencing the proposed biosensor were researched in detail, and the biosensor possessed excellent selectivity and good stability.

Key words: L-cysteine, nano-Prussian blue, glucose biosensor, nano-gold, chitosan