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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (19): 2174-2177. 上一篇    下一篇

研究简报

固定Fe 3＋的磁性微球分离富集鼠肝中铁结合蛋白的初步研究

方彩云a 王 胜a 刘清萍a 晏国全a
陆豪杰*,a,b,c 杨芃原*,a,b,c

  

  1. (a复旦大学化学系 上海 200433)
    (b复旦大学生物医学研究院 上海 200032)
    (c复旦大学教育部创新科学仪器工程中心 上海 200433)

  • 投稿日期:2008-02-04 修回日期:2008-05-12 发布日期:2008-10-14
  • 通讯作者: 杨芃原

Preliminary Study of Iron-binding Proteins in Rat Liver by Magnetic Microsphere Immobilized Iron Affinity Chromatography

FANG, Cai-Yun a WANG, Sheng a LIU, Qing-Ping a YAN, Guo-Quan a
LU, Hao-Jie *,a,b,c YANG, Peng-Yuan *,a,b,c
  

  1. (a Department of Chemistry, Fudan University, Shanghai 200433)
    (b Institute of Biomedical Sciences, Fudan University, Shanghai 200032)
    (c Education Ministry Engineering Center for Innovative Scientific-instrument, Fudan University, Shanghai 200433)
  • Received:2008-02-04 Revised:2008-05-12 Published:2008-10-14
  • Contact: YANG, Peng-Yuan

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采用键合Fe3＋的纳米材料分离富集了大鼠肝脏中的铁结合蛋白质组, 并进行了质谱分析. 在相同的起始富集蛋白质量以及相同的吸附和洗脱条件下, 键合了Fe3＋的磁性纳米材料比未键合金属离子的空白材料富集了更多的蛋白质, 经质谱鉴定得到42个蛋白质, 主要包括代谢酶类、呼吸链主要成员、氧化还原蛋白、转运蛋白、血红蛋白等.

关键词: 磁性纳米材料, 铁结合, 蛋白质组

Iron-binding proteome of rat liver were enriched by Fe3＋-immobilized silica nanomaterials and analyzed by HPLC-MS. More proteins were identified with enrichment of the Fe3＋-immobilized silica nanomaterials than those with normal silica nanomaterials when the starting proteins with the same amount and the same conditions of absorption and elution were used. Finally, 42 iron-binding proteins were identified, which were mainly referred to enzymes of metabolism, members of respiratory chain, proteins involved in oxidation-reduction, transporters and hemoglobins.

Key words: magnetic nanomaterial, iron-binding, proteome