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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (03): 263-268. 上一篇    下一篇

研究论文

核壳型Fe3O4/Au粒子的制备及其在表面等离子体子共振传感器中的应用

王健，王丽英，孙颖，朱晓楠，徐昊妍，毕宁，张寒琦，曹彦波，王兴华，宋大千   

  1. (吉林大学化学学院 长春 130012)
  • 投稿日期:2009-04-08 修回日期:2009-09-10 发布日期:2010-02-20
  • 通讯作者: 宋大千 E-mail:songdq@jlu.edu.cn
  • 基金资助:

    国家自然科学基金（20727003）

Preparation of Core/Shell Fe3O4/Au Nanocomposite and Its Application to Surface Plasmon Resonance Biosensor

Wang Jian Wang Liying Sun Ying Zhu Xiaonan Xu Haoyan Bi Ning Zhang Hanqi Cao Yanbo Wang Xinghua Song Daqian*   

  1. (College of Chemistry, Jilin University, Changchun 130012)
  • Received:2009-04-08 Revised:2009-09-10 Published:2010-02-20

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合成了核壳型Fe3O4/Au复合粒子, 并对其形貌、光学性质进行了表征. 通过外加磁场将Fe3O4/Au复合粒子与兔抗人IgG的偶联体固定于表面等离子体子共振(SPR)传感器的金基底膜上, 形成了Fe3O4/Au/抗IgG敏感膜. 与传统的通过巯基丙酸连接蛋白的方式相比, 磁场作用固定的Fe3O4/Au/抗IgG敏感膜制备简单, 易洗脱, 具有良好的再生性, 且在一定程度上提高了传感器的灵敏度. 并对人IgG进行了测定, 结果表明, 传感器对于浓度范围在1.25～20.00 μg•mL－1的人IgG有良好的信号响应.

关键词: Fe3O4/Au复合粒子, 表面等离子体子共振SPR, 人IgG

The synthesis and characterization of core/shell Fe3O4/Au nanocomposite were performed. Furthermore, its application to a surface plasmon resonance (SPR) biosensor was mainly studied. The Fe3O4/Au nanocomposite coupled with rabbit anti-human IgG (anti-IgG) was trapped on the Au film by magnetic force and the Fe3O4/Au/anti-IgG sensing membrane was prepared. There is not a covalent link between the membrane and Au film, which simplifies the immobilization of the antibody and enhances the sensitivity of biosensor. The SPR biosensor exhibits a satisfactory response for human IgG in the concentration range of 1.25～20.00 μg•mL－1 and shows good reproducibility.

Key words: Fe3O4/Au nanocomposite, surface plasmon resonance (SPR), human IgG