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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (03): 257-262. 上一篇    下一篇

研究论文

明胶固定辣根过氧化物酶制备H2O2传感器

屈建莹*，陈文静   

  1. (河南大学化学化工学院环境与分析科学研究所 开封 475004)
  • 投稿日期:2009-04-28 修回日期:2009-09-03 发布日期:2010-02-20
  • 通讯作者: 屈建莹 E-mail:qujy@henu.edu.cn；lilyqujy@yahoo.com.cn；chenwenjing128@yahoo.com.cn

Hydrogen Peroxide Biosensor Based on Gelatin Immobilizing Horseradish Peroxidase Qu Jianying* Chen Wenjing

Qu Jianying* Chen Wenjing   

  1. (Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004)
  • Received:2009-04-28 Revised:2009-09-03 Published:2010-02-20

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用明胶将辣根过氧化物酶(HRP)固定于多壁碳纳米管(MWNT)和茜素红(AR)修饰的玻碳(GC)电极上, 制成HRP生物传感器(HRP/AR/MWNT/GC), 然后在3%戊二醛(GA)中进行交联改性, 以克服明胶膜易溶胀的缺点, 并提高膜的稳定性. 同时详细探讨了该传感器对H2O2的响应性能, 并优化了实验条件. 结果表明, 该传感器对H2O2的线性响应范围为5.0×10－6～1.0×10－3 mol/L, 线性相关系数为0.9932, 检出限为1.0×10－7 mol/L, 且放于4 ℃环境30 d后, 峰电流值约为原来的72.1%. 该传感器响应快速, 灵敏度高, 且具有良好的重现性、稳定性及较长的使用寿命, 具有潜在的应用价值.

关键词: 多壁碳纳米管, 茜素红, 明胶, 辣根过氧化物酶, 戊二醛

A novel hydrogen peroxide biosensor was obtained by successfully immobilizing horseradish peroxidase (HRP) on a glassy carbon (GC) electrode, which was modified with multi-walled carbon nanotubes (MWNT) and alizarin red (AR). Then the biosensor was immerged in 3% glutaraldehyde (GA) in order to overcome the swelling and improve the stability of the gelatin membrane. The experimental condition was optimized, and the electrochemistry behavior of the HRP/AR/MWNT/GC biosensor in PBS (pH 7.0) and H2O2 was discussed. The biosensor displayed rapid response and expanded linear response range from 5.0×10－6 to 1.0×10－3 mol/L (R＝0.9932) with detection limit of 1.0×10－7 mol/L in PBS (pH 7.0), as well as acceptable preparation reproducibility and excellent stability.

Key words: multi-walled carbon nanotube, alizarin red, gelatin, horseradish peroxidase, glutaraldehyde