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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (07): 672-678. 上一篇    下一篇

研究论文

空壳纳米金修饰的新型DNA传感器

卜扬，杨清，孟琦，胡赢，黄杉生   

  1. (上海师范大学生命与环境科学学院 上海 200234)
  • 投稿日期:2009-06-10 修回日期:2009-09-29 发布日期:2010-04-14
  • 通讯作者: 黄杉生 E-mail:sshuang@shnu.edu.cn
  • 基金资助:

    863项目（编号2007AA06Z402），上海市科委项目（编号：0852510400），上海市重点学科资助项目（S30406），上海师范大学重点学科项目（DZL706）

A Novel DNA Biosensor Modified with Hollow Gold Nanoparticles

BO Yang, YANG Qing, MENG Qi, HU Ying, HUANG Sha-Sheng,   

  1. (Life and Environment Science College, Shanghai Normal University, Shanghai 200234)
  • Received:2009-06-10 Revised:2009-09-29 Published:2010-04-14

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被引次数

28 | 3

利用新型材料金纳米空球, 通过层层修饰的技术, 分别将壳聚糖、空壳纳米金、L-半胱氨酸、细胞色素c以及ssDNA探针修饰到玻碳电极表面, 制备了一种新型的DNA生物传感器. 以紫外及透射电子显微镜(TEM)表征了空壳纳米金, 以循环伏安法、阻抗谱图等电化学方法研究了传感器的特性, 通过原子力显微镜方法观察了该DNA生物传感器不同层之间的形态差异. 结果表明, 该修饰电极所吸附的ssDNA探针为1.672×10―10 mol•cm－2. 在指示剂柔红霉素的帮助下, DNA探针可与互补的DNA进行杂交, 借此以微分脉冲伏安法测定DNA.

关键词: 金纳米空球, ssDNA, 传感器

A novel DNA biosensor was fabricated by modifying ssDNA, cytochrome c, L-cysteine, novel material hollow gold nanoparticles and Chitosan on the surface of glassy carbon electrode. The hollow gold nanoparticles (HGNs) were characterized by violet spectroscopy and transmission electron microscopy. The behavior of the DNA biosensor was investigated by circle voltammetry, impedance spectrum. The morphologic differences among each layer of the DNA biosensor were also observed by atomic force microscope. Results revealed that the amount of adsorbed DNA was 1.672×10―10 mol•cm－2. It could be concluded that the modified electrode could be used to detect DNA in the event of hybridization with the indicator daunomycin by differential pulse voltammetry (DPV).

Key words: hollow gold nanoparticles, ssDNA, sensor