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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (17): 1719-1725. 上一篇    下一篇

研究论文

三聚氰胺与鯡鱼精DNA相互作用研究

沈昊宇*，朱凡，施炜，陈真，潘青青   

  1. (浙江大学宁波理工学院生物与化工分院 宁波 315100)
  • 投稿日期:2009-08-18 修回日期:2010-01-07 发布日期:2010-05-06
  • 通讯作者: 沈昊宇 E-mail:haoyushen2000@163.com
  • 基金资助:

    浙江省科技基础条件平台研究项目(2007F0045);宁波市自然科学基金资助项目(2009A610002)

Studies of Interaction between Melamine and Herring Sperm DNA

SHEN Hao-Yu, ZHU Fan, SHI Wei, CHEN Zhen, PAN Qing-Qing   

  1. (Ningbo Institute of Technology, Zhejiang University, Ningbo 315100)
  • Received:2009-08-18 Revised:2010-01-07 Published:2010-05-06
  • Contact: Shen Hao-Yu Shen E-mail:haoyushen2000@163.com

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1684

被引次数

35 | 3

采用UV、荧光光谱、电化学和粘度法等方法研究了三聚氰胺(MM)与鲱鱼精DNA的作用机制, 并采用凝胶电泳考察了MM对pBR322质粒DNA迁移作用的影响. 当pH为7.0时, 动力学参数的计算结果表明: 加入DNA前后MM电荷转移系数α分别为0.27和0.26, 速率常数ks分别为5.58 s-1和5.65 s－1; 热力学研究表明MM与DNA的结合常数K约为105 mol－1•L, MM与DNA可能是以沟槽式相结合.

关键词: 三聚氰胺, 鲱鱼精DNA, 作用方式, 动力学常数, 热力学常数

UV-Vis spectroscopy, fluorescence spectroscopy, cyclic voltametry (CV), and viscosimetric methods were applied to investigate the mechanism of the interaction between melamine (MM) and herring sperm DNA. The effect of MM on the migration rate of pBR322 plasmid DNA was investigated by gel electrophoretic method as well. The results showed that for the system of pH at 7.0, the charge transfer coefficients (α) were 0.27 and 0.26, with the velocity constant (ks) at 5.58 s-1 and 5.65 s－1 for the MM before and after DNA added, respectively. The interaction between MM and DNA may be realized via groove combined mode. The thermodynamic formulation constant (K) was ca. 105 mol－1•L.

Key words: melamine, herring sperm DNA, interaction mode, dynamic constant, thermodynamic formulation constant