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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (08): 973-980. 上一篇    下一篇

研究论文

加替沙星印迹聚合物微球的合成及特异吸附性能

朱桂芬1，高燕哺1，高霞1,2，樊静*,1   

  1. (1河南师范大学化学与环境科学学院 黄淮水环境与污染防治省部共建教育部重点实验室 河南省环境污染控制重点实验室 新乡 453007)
    (2新乡医学院公共卫生学系 新乡 453003)
  • 投稿日期:2010-08-01 修回日期:2010-11-03 发布日期:2010-12-31
  • 通讯作者: 樊静 E-mail:fanjing@henannu.edu.cn
  • 基金资助:

    国家自然科学基金项目;河南省基础与前沿技术研究计划

Preparation and Separation Properties of Gatifloxacin Molecularly Imprinted Polymer Microsphere

Zhu Guifen1 Gao Yanbu1 Gao Xia1,2 Fan Jing*,1   

  1. (1 School of Chemistry and Environmental Science, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control of Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, Henan Normal University, Xinxiang 453007)
    (2 School of Public Health, Xinxiang Medical University, Xinxiang 453003)
  • Received:2010-08-01 Revised:2010-11-03 Published:2010-12-31
  • Contact: jing fan E-mail:fanjing@henannu.edu.cn

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1342

被引次数

14

采用沉淀聚合法制备了加替沙星分子印迹聚合物(MIP)微球, 对其形貌和结构进行表征, 并对聚合反应条件进行优化|采用静态吸附试验研究聚合物对模板分子及其结构类似物的吸附行为和选择性识别特性. 结果表明, 在最佳实验条件下, 40 min印迹聚合物即可达到对底物的吸附平衡|在印迹聚合物中存在两类结合位点, 特异性和非特异性结合位点的最大吸附量分别为363和458 μmol/g|与化学组成相同的非印迹聚合物(NIP)相比, MIP对结构相似的喹诺酮类抗生素具有良好的选择吸附性能|该法制备的MIP微球易于再生, 放置3个月以后, 仍能够对喹诺酮类抗生素进行有效识别.

关键词: 加替沙星, 分子印迹聚合物, 特异吸附

The gatifloxacin molecularly imprinted polymer (MIP) microsphere was prepared via precipitation polymerization. The morphologies and structures of the microsphere were characterized, and the polymerization reaction conditions were optimized. The static adsorption experiments were performed to investigate the adsorption properties and selective recognition characteristics of the polymer for the template molecule and its structural analogues. The results showed that the adsorption equilibrium of MIP for the substrates was achieved in 40 min under the optimized conditions. There existed two types of binding sites in the MIP, and the maximum adsorption capacities of specific and non-specific sites were 363 and 458 µmol/g, respectively. Compared with the corresponding non-imprinted polymer, the MIP exhibited better selective adsorption performance for quinolone antibiotics. The MIP prepared by precipitation polymerization was easy to regeneration, and it still could recognize effectively quinolone antibiotics 3 months later.

Key words: gatifloxacin, molecularly imprinted polymer, specific adsorption