化学学报 ›› 2012, Vol. 70 ›› Issue (20): 2137-2143.DOI: 10.6023/A12070461 上一篇    下一篇

研究论文

寡聚芴纳米粒子的制备、表征与细胞成像应用

温泉山, 唐红伟, 杨高买, 刘礼兵, 吕凤婷, 杨琼, 王树   

  1. 中国科学院化学研究所 有机固体实验室 北京 100190
  • 投稿日期:2012-07-24 发布日期:2012-09-11
  • 通讯作者: 刘礼兵, 王树 E-mail:wangshu@iccas.ac.cn; liulibing@iccas.ac.cn
  • 基金资助:

    项目受国家自然科学基金(Nos. 21033010, 21021091)和国家重点基础研究发展计划(Nos. 2011CB932302, 2011CB808400)资助.

Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging

Wen Quanshan, Tang Hongwei, Yang Gaomai, Liu Libing, Lv Fengting, Yang Qiong, Wang Shu   

  1. Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190
  • Received:2012-07-24 Published:2012-09-11
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos. 21033010, 21021091) and the Major Research Plan of China (Nos. 2011CB932302, 2011CB808400).

利用Suzuki偶联反应合成了疏水性寡聚芴分子OF, 并对其在氯仿溶液中的紫外吸收和荧光光谱进行了表征, 表明其具有较大的摩尔吸光系数(1.08×105 mol-1·L·cm-1)和高荧光量子产率(96%). OF分子分散到水中可形成纳米粒子, 动态光散射实验表明其粒径大小约为230 nm. 该纳米粒子在水相中仍保持了较大的摩尔吸光系数以及高的荧光量子产率. 我们利用MTT的方法对OF纳米粒子对人肺癌A549细胞的毒性进行了测试, 结果表明其具有低的细胞毒性, 因此可以用于细胞成像. 共聚焦激光扫描显微镜成像结果显示OF纳米粒子主要分布在细胞质中, 特别是在近核区域周围. 与溶酶体染料Lyso Tracker Red共定位结果表明OF纳米粒子主要存在于溶酶体中, 因此可以用于对溶酶体的特异性荧光成像.

关键词: 共轭寡聚物, 纳米粒子, 细胞成像, 溶酶体

A new hydrophobic oligofluorene (OF) is synthesized via Suzuki cross-coupling reaction. The absorption and fluorescence spectra are measured in chloroform with high molar absorption coefficient (1.08?105 mol-1稬穋m-1) and fluorescence quantum yield (0.96). OF is dispersed into water to form nanoparticles with average size of 230 nm from dynamic light scattering (DLS) measurement. The nanoparticles in water still possess high molar absorption coefficient and fluorescence quantum yield. The quantitative cytotoxicity of OF nanoparticles to human lung cancer (A549) cells is measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, showing that OF nanoparticles exhibit low cellular toxicity. OF nanoparticles can be utilized for imaging of live A549 cells. Confocal laser scanning microscopy demonstrates that OF nanoparticles are exclusively located in the cytoplasm, especially around the perinuclear region. OF nanoparticles exhibit good overlap staining with lysosome-specific dye (Lyso Tracker Red), which demonstrates that OF nanoparticles could be used for lysosome-specific imaging.

Key words: conjugated oligomer, nanoparticles, cellular imaging, lysosome