化学学报 ›› 1965, Vol. 31 ›› Issue (6): 482-485. 上一篇    下一篇

常山碱的比色测定法

李祿先, 区嘉煒, 謝淑敏   

  1. 中国科学院微生物研究所
  • 投稿日期:1964-10-06 发布日期:2013-06-03

A COLORIMETRIC METHOD FOR THE DETERMINATION OF FEBRIFUGINE AND ISOFEBRIFUGINE

LEE LOU-SIEN, OU CHIA-WEI, HSIE SHu-MIN   

  1. Institute of Microbiology, Academia Sinica
  • Received:1964-10-06 Published:2013-06-03

根据常山碱和异常山碱分子中的喹唑酮能碱水解而形成邻氨基苯甲酸的特点,制订常山植株中碱类含量的比色方法。邻氨基苯甲酸可与经重氮化的N-1-萘基-乙二胺偶合后,于530毫微米处读光密度。此法的灵敏度以光密度值/微克异常山碱/毫升比色液表示为0.111。用此法检查通常用的氯仿萃取法,得知其所回收的常山碱类尚不及实际含量之半。

Dichroa febrifuga has been employed by Chinese physicians as a medical cure for malaria since ancient times. Up to the present, no satisfactory quantitative method for the estimation of its effective components, febrifugine and isofebrifugine, is available. The present work presents a colorimetric method based on the principle that o-amino-benzoic acid is produced by the alkaline hydrolysis of the quinazolone structure of the febrifugine or isofebrifugine molecule, which can be determined colorimetrically by cou- pling with N-1-naphthyl ethylene diamine after diazotization. The optical density-concentration curve of the isofebrifugine hydrolysate was found to coincide with that of pure o-aminobenzoic acid and it did follow Beer's Law. An analytical procedure was established after a systematic examination of the conditions of hydrolysis and colour formation. The sensitivity of detection as expressed in the optical density at 530 mμ per microgram of isofebrifugine per millilitre of coloured solution was found to be 0.111/μg/ml.