外源镧(Ⅲ)对辣根过氧化物酶的结构的影响

化学学报 ›› 2004, Vol. 62 ›› Issue (14): 1318-1322. 上一篇下一篇

外源镧(Ⅲ)对辣根过氧化物酶的结构的影响

夏炳乐, 彭敦耕, 郑晓云, 肖厚荣, 盛良全, 刘清亮

中国科学技术大学化学系, 合肥, 230026

投稿日期:2003-05-07 修回日期:2004-03-17 发布日期:2014-02-17
通讯作者: 夏炳乐,E-mail:xiabl@ustc.edu.cn;彭敦耕,E-mail:dgpeng@ustc.edu E-mail:xiabl@ustc.edu.cn;dgpeng@ustc.edu
基金资助:
国家烟草专卖局科研基金(No.1002001041)资助项目.

Effect of Extraneous Lanthanum(Ⅲ) on the Structure of Horseradish Peroxidase

XIA Bing-Le, PENG Dun-Geng, ZHENG Xiao-Yun, XIAO Hou-Rong, SHENG Liang-Quan, LIU Qing-Liang

Department of Chemistry, University of Science and Technology of China, Hefei 230026

Received:2003-05-07 Revised:2004-03-17 Published:2014-02-17

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摘要/Abstract

运用荧光光谱、傅立叶变换红外光谱以及曲线拟合等技术研究了镧离子对辣根过氧化物酶的结构的影响.结果表明荧光增强的原因是由于镧离子的加入使镧离子和酶结合,酶的二级结构发生变化,导致荧光发色基团所处的微环境也相应变化;并运用它推测了镧离子对该酶的激活作用机制.

关键词: 辣根过氧化物酶, 镧(Ⅲ), 结合, 微环境, 二级结构

Through the fluorescence spectra, FT-IR spectra and curve-fit, the effect of lanthanum(Ⅲ) on horseradish peroxidase (HRP) was studied.It is concluded that the reason of the stimulation is due to the binding of La(Ⅲ) to HRP, which induced the changes of the enzyme's secondary structure and the microenvironment of the fluorophore, while adding La(Ⅲ) into the HRP.The activation mechanism of La(Ⅲ) to the HRP was also discussed.

Key words: horseradish peroxidase, lanthanum(Ⅲ), binding, microenvironment, secondary structure

引用此文

夏炳乐, 彭敦耕, 郑晓云, 肖厚荣, 盛良全, 刘清亮. 外源镧(Ⅲ)对辣根过氧化物酶的结构的影响[J]. 化学学报, 2004, 62(14): 1318-1322.

XIA Bing-Le, PENG Dun-Geng, ZHENG Xiao-Yun, XIAO Hou-Rong, SHENG Liang-Quan, LIU Qing-Liang. Effect of Extraneous Lanthanum(Ⅲ) on the Structure of Horseradish Peroxidase[J]. Acta Chimica Sinica, 2004, 62(14): 1318-1322.

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参考文献

[1] Szurdoki, F.; Michael, K. L.; Walt, D. R. Anal Biochem.2001, 291(2), 219.
[2] Stefan, R.-I.; Nejem, R. M.; van Staden, J. F. Sens. Actuators, B: Chem. 2003, 94(3), 271.
[3] Felts, P. A.; Smith, K. J. Neuroscience 1996, 75(2), 643.
[4] Xia, B.-L.; Peng, D.-G.; Liu, Q.-L. Chem. J. Internet 2003, 5, 0510001pc, http://web. chemistrymag. org/cji/2003/051001pc. htm (in Chinese).(夏炳乐,彭敦耕,刘清亮,国际网上化学学报,2003,5,0510001pc. )
[5] Wang, S.-Y.; Xu, X.-L.; Liu, Q.-L.; Xie, Y.-S. Prog.Chem. 2001, 13, 257 (in Chinese).(王守业,徐小龙,刘清亮,解永树,化学进展,2001,13,257. )
[6] Xie, J.-A.; Shen, Y.-H.; Mao, J.-Q. Spectrosc. Spectral. Anal. 2001, 21, 347 (in Chinese).(谢建安,沈玉华,茆建强,光谱学与光谱分析,2001,21,347. )
[7] Holzbaur, I. E.; English, A. M.; Ismail, A. A. Biochemistry 1996, 35, 5488.
[8] Jiang, J.-G.; Wang, Z.-X.; Liu, C.-W.; Liu, D.-J.; Yang, X.-R.; Dong, S.-J. Chem. J. Chin. Univ. 2001, 22, 1131 (in Chinese).(蒋俊光,王振新,刘长伟,刘殿骏,杨秀荣,董绍俊,高等学校化学学报,2001,22,1131.)
[9] Chen, C.-X.; Liu, J.; Luo, D. Biochemistry Experiment,University of Science and Technology of China Press, Hefei, 1994, p. 279.(陈曾燮,刘兢,罗丹,生物化学实验,中国科学技术大学出版社,合肥,1994,p.279.)
[10] Zhang, L.-X.; Zhang, T.-F.; Li, L.-Y. Experiments and Methods in Biochemistry, 2nd ed., Higher Education Press, Beijing, 1997, p. 188.(张龙翔,张庭芳,李令媛,生化试验与方法(第二版),高等教育出版社,北京,1997,p.188.)
[11] Du, X.-L.; Li, R.-C.; Wang, K. Sci. Bull. 2001, 46, 394 (in Chinese).(杜秀莲,李荣昌,王夔,科学通报,2001,46,394.)
[12] Chou, J.; Du, X.-G.; Feng, Y.-Y.; Yong, X.-J.; Lu, T.H. Chin. J. Anal. Chem. 2001, 29, 219 (in Chinese).(俞菊,杜江燕,冯玉英,杨秀娟,陆天虹,分析化学,2001,29,219.)
[13] Tao, W.-S.; Li, W.; Jiang, Y.-M. The Molecular Foundation of Protein, Higher Education Press, Beijing, 1995 (in Chinese).(陶慰孙,李惟,姜涌明,蛋白质分子基础,高等教育出版社,北京,1995.)
[14] Li, J.-Z. Bioinorganic Chemistry of Rare Earth Elements, Science Press, Beijing, 1995 (in Chinese).(倪嘉瓒,稀土生物无机化学,科学出版社,北京,1995.)
[15] Yang, P.; Gao, F. The Principles of Bioinorganic Chemistry,Science Press, Beijing, 2002 (in Chinese).(杨频,高飞,生物无机化学原理,科学出版社,北京,
[16] Yang, P.; Yang, M.-M.; Yang, B.-S. Chin. J. Chem. 1996, 14(2), 109.
[17] Krimm, S.; Bandekar, J. Adv. Protein Chem. 1986, 38, 181.
[18] Haris, P. L.; Chapman, D. Biopolymers 1995, 37, 251.
[19] Strickland, E. H.; Kay, E.; Shannon, L. M.; Horwitz, J. J. Biol. Chem. 1968, 243, 3560.