建立了一种新的电化学免疫传感器方法, 将多壁碳纳米管(MWCNTs)和室温离子液体1-丁基-3-甲基咪唑四氟硼酸盐([BMIM]BF₄)复合物, 和偶联了牛血清蛋白(BSA)的莱克多巴胺抗原, 使用Nafion固定在电极上, 利用莱克多巴胺抗体和抗原之间特定反应的竞争模式, 以K₃Fe(CN)₆为探针, 通过循环伏安法和差分脉冲伏安法监测免疫反应, 对溶液中莱克多巴胺的浓度进行检测. 线性范围宽(1～1500 ng/mL), 检测限可低至0.3 ng/mL. 同时, 我们对猪饲料实际样品进行测定, 回收率令人满意.

We have developed a new method of electrochemical immunosensor for the determination of ractopamine (RAC). The modified electrodes were prepared as follows. First, the multi-walled carbon nanotubes and ionic liquid composites were cast on glassy carbon electrode (GCE), and then the ractopamine-bovine serum albumin antigens were cast on GCE. At last, we dipped a few Nafion onto the electrode. Cyclic voltammetry and differential pulse voltammetry were employed to monitor the fabrication steps of immunoreaction system in solutions containing K₃Fe(CN)₆ as redox probes. The immunosensor demonstrates excellent RAC detection features with a broad linear range of 1 to 1500 ng/mL and a detection limit of 0.3 ng/mL. The tests with spiked animal feed samples showed excellent recovery for ractopamine.